o:34668 Comparative analysis of galectin-1, galectin-3, and galectin-8 signatures in normal and choriocarcinoma trophoblast cell lines Komparativna analiza otisaka galektina-1, galektina-3 i galektina-8 u normalnim i horiokarcinomskim trofoblastnim ćelijskim linijama en ABSTRACT Galectins are found in the placenta during both normal and pathological conditions. Specifically, galectins -1, -3, and -8 have been identified in extravillous trophoblast cells, with galectins -1, and -3 playing notable roles in trophoblast cell function. Altered expression profiles of galectins are linked to various cancers. This study explores the expression and localization of galectins -1, -3, and 8 in a normal extravillous HTR-8/SVneo cell line and two choriocarcinoma cell lines, JAR and JEG-3. Expression levels of these galectins at both mRNA and protein levels were evaluated using PCR, qPCR, and Western blotting, while their cellular localization and distribution were assessed through immunocytochemistry, subcellular fractionation, and Western blotting. Galectins -1, -3, and -8 were expressed at lower levels in JAR and JEG-3 cells compared to HTR-8/SVneo cells. Additionally, different splice variants of galectin-8 were detected across all cell lines. Galectin-1 was predominantly localized at the plasma membrane. Notable differences in galectin-3 localization and distribution were observed between HTR-8/SVneo cells and choriocarcinoma cells. These findings highlight variations in the expression, staining patterns, and subcellular distribution of galectins -1, -3, and -8, suggesting their potential involvement in the pathology of choriocarcinoma. galectin-1, galectin-3, galectin-8, extravillous trophoblast, choriocarcinoma cells 1552099 10.2298/ABS240605022J 2024-09-24T08:59:29.592Z 44 yes 46 Milica Jovanović Krivokuća University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia doktor bioloških nauka 0000-0001-8292-4853 Žanka Bojić-Trbojević University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia doktor bioloških nauka person 0000-0001-5619-1027 Ljiljana Vićovac doktor bioloških nauka person 0000-0002-2744-415X Ivana Grubiša Department of Human Genetics, Zvezdara University Medical Center, University of Belgrade, Belgrade, Serbia person Aleksandra Vilotić University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia doktor bioloških nauka person 0000-0002-0898-5401 Danica Ćujić University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia doktor medicinskih nauka-farmacija person 0000-0002-6145-3850 Janko Legner University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia person 0000-0003-3720-1933 application/pdf 2178028 https://phaidrabg.bg.ac.rs/o:34668 no yes 16 70 92000001 11A29 11A2901 11A29 11A2910 Archives of Biological Sciences Serbian Biological Society 2024