o:34488 Gestational diabetes is associated with an increased expression of miR-27a in peripheral blood mononuclear cells Gestacijski dijabetes je asociran sa povećanom ekspresijom miR-27a u mononuklearnim ćelijama periferne krvi en ABSTRACT Background: Dysregulation of microRNA-based mechanisms is associated with various human pathologies, including gestational diabetes mellitus (GDM), suggesting they may be potential diagnostic and/or prognostic biomarkers of GDM. Methods: The expression of miR-340-5p, miR-27a-3p and miR 222-3p in peripheral blood mononuclear cells (PBMCs) obtained from patients with GDM (n = 42) and healthy controls (n = 34) were evaluated, together with their correlation to the clinical parameters of participants and their newborns. Expression of the selected microRNAs was quantified by quantitative real-time polymerase chain reaction (qPCR), after reverse transcription with microRNA-specific stem-loop primers. Results: The expression of miR-27a-3p was significantly higher in patients with GDM than in controls (p = 0.036), whereas no significant difference between groups was found for the other two tested microRNAs. The expression level of miR-27a-3p in GDM patients was found to negatively correlate with the number of erythrocytes, concentration of haemoglobin, haematocrit, and low- and high-density lipoprotein (LDL/HDL) ratio, and positively with the concentration of glycated haemoglobin (HbA1c). In the case of miR-222-3p, a negative correlation between its expression and the concentration of cholesterol, LDL and LDL/HDL ratio was found only in healthy pregnant women. The expression level of miR-340-5p negatively correlated with erythrocyte count, haemoglobin concentration and haematocrit in GDM patients, as well as with the concentration of cholesterol, LDL and LDL/HDL ratio in healthy women. Conclusions: The results obtained illustrate the potential of PBMC-derived microRNA miR-27a-3p to serve as a diagnostic biomarker of GDM. On the other hand, MiR-27a and miR-340 may help in assessing the metabolic status relevant for pregnancy. "Accepted version" gestational diabetes, miR-27a, miR-222, miR-340, PBMC 1552099 10.1007/s40291-022-00591-5 2024-08-16T12:51:06.930Z 44 yes 46 Ognjen Radojičić University Clinic for Gynecology and Obstetrics "Narodni Front", Belgrade, Serbia Olgica Nedić University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia doktor biohemijskih nauka person 0000-0003-2042-0056 Željko Miković University Clinic for Gynecology and Obstetrics "Narodni Front", Belgrade, Serbia person Vesna Mandić Marković University of Belgrade, Medical School, Serbia person Nikola Gligorijević University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia doktor biohemijskih nauka person 0000-0002-8691-2486 Dragana Robajac University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia doktor bioloških nauka person 0000-0002-6423-4645 Zorana Dobrijević University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia doktor bioloških nauka person 0000-0002-4652-7719 application/pdf 935581 https://phaidrabg.bg.ac.rs/o:34488 no yes 1 70 92000001 11A29 11A2905 11A32 Molecular Diagnosis & Therapy 26 4 421 435 Springer 2022