application/pdf 474910 bytes http://creativecommons.org/licenses/by-nc-sa/4.0/legalcode eng Čutović, Natalija Batinić, Petar Pirković, Andrea Plećić, Ana Petrović, Predrag Jovanović, Aleksandra Vaccinium myrtillus leaf extract-loaded liposomes: the influence of UV irradiation 2024 Vaccinium myrtillus leaf extract-loaded liposomes: the influence of UV irradiation Due to the present phytochemicals (phenolic acids, flavonoids, and tannins), the extracts of Vaccinium myrtillus leaf were shown to scavenge free radicals in vitro, to enhance glutamate decarboxylase gene expression in dermal fibroblasts, resulting in the stimulation of cell growth and synthesis of hyaluronic acid and glutathione, to inhibit collagenase and elastase, enzymes responsible for the ragging and wrinkled nature of skin, to decrease the melanin content in B16 melanoma cells, and to suppress the release of histamine from mast cells. With the aim to improve the stability and bioavailability of the extract compounds, V. myrtillus leaf extract was encapsulated in the liposomes, and the influence of the UV irradiation on the particle size, polydispersity index (PDI), zeta potential, and mobility of obtained liposomes was investigated. The liposomal particles were prepared using the proliposome method and 4 g of phospholipids, 50 mL of V. myrtillus ethanol extract, and 20 mL of ultrapure water. Vesicle size, PDI, zeta potential, and mobility were measured by photon correlation spectroscopy before and after UV irradiation. The size and PDI did not change after UV irradiation and amounted to 5508.67±56.58 nm and 0.249±0.047, respectively. On the other hand, the zeta potential (as a parameter of the stability of the system) and mobility possessed low values (absolute values) at the beginning and additionally significantly changed in the UV-treated sample (-3.93±0.10 mV and -0.315±0.016 µmcm/Vs, respectively). The beneficial effects of biologically active V. myrtillus leaf phytochemicals on human and animal health, as well as their sensitivity, particularly under UV irradiation, encourage their encapsulation in liposomes. However, future experiments should be focused on the improvement of the stability of V. myrtillus extract-loaded liposomes, i.e., increasing the zeta potential value. info:eu-repo/semantics/conferenceProceedings encapsulation, liposomal particles, Vaccinium myrtillus, UV irradiation https://phaidrabg.bg.ac.rs/o:34259 ISBN: 978-86-7834-438-1