o:34258 Optimizing parameters related to the phenolic and flavonoid contents of Steepe peony leaves en The current study had two objectives: (1) the determination of the total phenolic content (TPC) and total flavonoid content (TFC) of steppe peony leaf extracts prepared in two different extraction methods, maceration using conventional orbital shaker (MCOS) and ultrasound-assisted extraction (UAE) and (2) optimization of the extraction process parameters via varying solvent type (aqueous, and ethanolic (50% and 70%, v/v), time of extraction (5, 15, 30, and 60 min), and solid-to-solvent ratio (1:10, 1:20, 1:30, 1:40, and 1:50). The total phenolics were extracted most effectively by UAE (411,3 mg gallic acid equivalents (GAE)/mL of raw extract). The MCOS gave lower values, approximately 112,9 mg GAE/mL of raw extract. Total flavonoids content were ranged from 3,0 to 20,0 mg catechin equivalents (CE)/mL of raw extract for UAE, and from 2,98 to 22,87 mg CE/mL of raw extract for MCOS. For all tested analytes, it was observed that a concentration of ethanol of 50% (v/v), a time of extraction of 15 min, and the solid-to-solvent ratio of 1:20 were the most effective for extracting bioactive substances (phenolics and flavonoids) from Steppe peony. phenolics, colorimetric determination, gallic acid equivalents, conventional and „green“ extraction 1552100 978-86-7834-438-1 2024-07-10T08:52:12.395Z 44 yes 46 Petar Batinić Institute for Medicinal Plants Research “Dr Josif Pančić”, Belgrade, Serbia Natalija Cutovic Institute for Medicinal Plants Research “Dr Josif Pančić”, Belgrade, Serbia person Aleksandra A. Jovanović University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia doktor tehnoloških nauka person 0000-0001-5394-0125 Tatjana Marković Institute for Medicinal Plants Research “Dr Josif Pančić” Belgrade, Serbia person application/pdf 474910 https://phaidrabg.bg.ac.rs/o:34258 no yes 19 70 92000004 11A29 Optimizing parameters related to the phenolic and flavonoid contents of Steepe peony leaves 37 Book of Abstracts 3rd UNIFood International Conference – UNIFood2024 Belgrade, Serbia 2024