https://phaidrabg.bg.ac.rs/o:34258 ISBN: 978-86-7834-438-1 Optimizing parameters related to the phenolic and flavonoid contents of Steepe peony leaves phenolics, colorimetric determination, gallic acid equivalents, conventional and „green“ extraction Batinić, Petar Cutovic, Natalija Jovanović, Aleksandra A. Marković, Tatjana http://creativecommons.org/licenses/by-nc-sa/4.0/legalcode Optimizing parameters related to the phenolic and flavonoid contents of Steepe peony leaves eng 2024 The current study had two objectives: (1) the determination of the total phenolic content (TPC) and total flavonoid content (TFC) of steppe peony leaf extracts prepared in two different extraction methods, maceration using conventional orbital shaker (MCOS) and ultrasound-assisted extraction (UAE) and (2) optimization of the extraction process parameters via varying solvent type (aqueous, and ethanolic (50% and 70%, v/v), time of extraction (5, 15, 30, and 60 min), and solid-to-solvent ratio (1:10, 1:20, 1:30, 1:40, and 1:50). The total phenolics were extracted most effectively by UAE (411,3 mg gallic acid equivalents (GAE)/mL of raw extract). The MCOS gave lower values, approximately 112,9 mg GAE/mL of raw extract. Total flavonoids content were ranged from 3,0 to 20,0 mg catechin equivalents (CE)/mL of raw extract for UAE, and from 2,98 to 22,87 mg CE/mL of raw extract for MCOS. For all tested analytes, it was observed that a concentration of ethanol of 50% (v/v), a time of extraction of 15 min, and the solid-to-solvent ratio of 1:20 were the most effective for extracting bioactive substances (phenolics and flavonoids) from Steppe peony. application/pdf 474910 bytes info:eu-repo/semantics/conferenceProceedings