o:34255 Total polyphenol and protein contents in different Fumaria officinalis extracts en Fumaria officinalis L. (Fumariaceae) is a component of various phytotherapeutic formulations in the European ethnobotany used in hepatobiliary dysfunction, illnesses of gastrointestinal and urogenital tracts, cancer, rheumatism, high blood pressure, and skin disorders. Various extraction techniques can isolate bioactive compounds from plant material; however, they differ in terms of extraction speed and efficiency, the yield of target molecules, solvent and energy consumption. Therefore, in the present study, microwave- and ultrasound-assisted extractions (MAE and UAE, respectively) and two different solid-to-solvent ratios (1:20 and 1:30) were used for polyphenol and protein extractions from F. officinalis herba. MAE process was performed in a microwave reactor for 2 min, while UAE was done in an ultrasound bath for 15 min. The total polyphenol content (TPC) was determined in the Folin-Ciocalteu assay, while the total protein yield was measured in the Bradford protein assay. The TPC varied in a range of 15.2 to 23.7 mg gallic acid equivalents (GAE)/g of dried plant material (dw), achieving the highest value in the extract prepared using MAE and a 1:30 ratio, followed by MAE and a 1:20 ratio and UAE and a 1:30 ratio, while the lowest polyphenol yield was obtained using UAE and a 1:20 ratio. The concentration of proteins in F. officinalis extracts follows the trend: MAE and a 1:30 ratio (68.3 mg/g of dw) > MAE at a 1:20 ratio (67.2 mg/g of dw) and UAE at a 1:30 ratio (67.5 mg/g of dw) > UAE and a 1:20 ratio (64.3 mg/g of dw). Due to significantly higher polyphenol and protein yields, F. officinalis extract prepared using MAE (as a more rapid technique) and a higher employed level of solid-to-solvent ratio (1:30) can be potentially implemented in different food, functional food, dietetic supplement, or pharmaceutical formulations. Fumaria officinalis, extraction, polyphenols, proteins 1552100 978-86-7834-438-1 2024-07-10T08:22:12.542Z 44 yes 46 Rabiea Ashowen Ahmoda University of Belgrade Faculty of Technology and Metallurgy Milena Milošević University of Belgrade, Institute of Chemistry, Technology and Metallurgy, National Institute of the Republic of Serbia, Serbia person Petar Batinić Institute for Medicinal Plants Research “Dr Josif Pančić”, Belgrade, Serbia person Andrea Pirković University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia doktor bioloških nauka person 0000-0002-1182-4895 Aleksandar Krstić “VINČA” Institute of Nuclear Sciences - National Institute of the Republic of Serbia, University of Belgrade, Serbia person Aleksandar Marinković University of Belgrade, Faculty of Technology and Metallurgy, Serbia person Aleksandra A. Jovanović University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia doktor tehnoloških nauka person 0000-0001-5394-0125 application/pdf 474910 https://phaidrabg.bg.ac.rs/o:34255 no yes 19 70 92000004 11A24 11A28 11A41 11A29 Total polyphenol and protein contents in different Fumaria officinalis extracts 92 Book of Abstracts 3rd UNIFood International Conference – UNIFood2024 Belgrade, Serbia 2024