https://phaidrabg.bg.ac.rs/o:34255 ISBN: 978-86-7834-438-1 Total polyphenol and protein contents in different Fumaria officinalis extracts Ahmoda, Rabiea Ashowen Milošević, Milena Batinić, Petar Pirković, Andrea Krstić, Aleksandar Marinković, Aleksandar Jovanović, Aleksandra A. Fumaria officinalis, extraction, polyphenols, proteins Total polyphenol and protein contents in different Fumaria officinalis extracts http://creativecommons.org/licenses/by-nc-sa/4.0/legalcode eng Fumaria officinalis L. (Fumariaceae) is a component of various phytotherapeutic formulations in the European ethnobotany used in hepatobiliary dysfunction, illnesses of gastrointestinal and urogenital tracts, cancer, rheumatism, high blood pressure, and skin disorders. Various extraction techniques can isolate bioactive compounds from plant material; however, they differ in terms of extraction speed and efficiency, the yield of target molecules, solvent and energy consumption. Therefore, in the present study, microwave- and ultrasound-assisted extractions (MAE and UAE, respectively) and two different solid-to-solvent ratios (1:20 and 1:30) were used for polyphenol and protein extractions from F. officinalis herba. MAE process was performed in a microwave reactor for 2 min, while UAE was done in an ultrasound bath for 15 min. The total polyphenol content (TPC) was determined in the Folin-Ciocalteu assay, while the total protein yield was measured in the Bradford protein assay. The TPC varied in a range of 15.2 to 23.7 mg gallic acid equivalents (GAE)/g of dried plant material (dw), achieving the highest value in the extract prepared using MAE and a 1:30 ratio, followed by MAE and a 1:20 ratio and UAE and a 1:30 ratio, while the lowest polyphenol yield was obtained using UAE and a 1:20 ratio. The concentration of proteins in F. officinalis extracts follows the trend: MAE and a 1:30 ratio (68.3 mg/g of dw) > MAE at a 1:20 ratio (67.2 mg/g of dw) and UAE at a 1:30 ratio (67.5 mg/g of dw) > UAE and a 1:20 ratio (64.3 mg/g of dw). Due to significantly higher polyphenol and protein yields, F. officinalis extract prepared using MAE (as a more rapid technique) and a higher employed level of solid-to-solvent ratio (1:30) can be potentially implemented in different food, functional food, dietetic supplement, or pharmaceutical formulations. 2024 application/pdf 474910 bytes info:eu-repo/semantics/conferenceProceedings