o:34253 Antioxidant capacity of silibinin-loaded liposomes en Silibinin is the major active constituent of silymarin, a standardized extract of milk thistle seeds. Silibinin has been used traditionally as a chemopreventive and therapeutic agent in human lung cancer. It also possesses hepatoprotective, antioxidant, hypocholesterolemic, antitumor, cardioprotective, neuroprotective, and antiviral activities. However, its application is limited due to poor water solubility, intestinal resorption, and low bioavailability. Liposomes, as non-toxic, biodegradable, and biocompatible lipid carriers, can provide controlled delivery of bioactive components and their protection from degradation caused by light, oxygen, UV irradiation, different pH values, and enzymes. Additionally, phospholipids from the liposomes do not provoke a reaction with taste receptors, and, therefore, the liposomal bilayer is an appropriate carrier for covering the unpleasant taste of numerous polyphenols. With the aim of investigating the antioxidant potential of silibinin-loaded liposomes after different technological procedures (UV irradiation and lyophilization), two antioxidant assays were employed (ABTS and DPPH tests). Liposomes with silibinin were prepared using the proliposome method and phospholipids. According to the results of the ABTS test, the antioxidant activity of pure silibinin was 0.769 µmol Trolox equivalent (TE)/mL, while the antioxidant potential was lower after the encapsulation in liposomes; 0.548 µmol TE/mL after preparation, 0.549 µmol TE/mL after UV irradiation, and 0.436 µmol TE/mL after lyophilization. Furthermore, the DPPH radical scavenging activity was 20.97% for pure silibinin, 22.48% immediately after the liposomal preparation, 22.24% after UV irradiation, and 18.73% after lyophilization. As can be seen, UV irradiation did not cause significant changes in the overall antioxidant potential of silibinin-loaded liposomes. Nevertheless, lyophilization significantly decreased the radical scavenging activity of the liposomes. Considering that the two used antioxidant assays are based on different principles and reactions, the obtained results provide good insight into the overall antioxidant activity of silibinin-loaded liposomes. antioxidant capacity, encapsulation, liposomes, proliposome method, silibinin 1552100 978-86-7834-438-1 2024-07-10T08:02:14.209Z 44 yes 46 Amjed Karkad University of Belgrade Faculty of Technology and Metallurgy Petar Batinić Institute for Medicinal Plants Research “Dr Josif Pančić”, Belgrade, Serbia person Milena Milošević University of Belgrade, Institute of Chemistry, Technology and Metallurgy, National Institute of the Republic of Serbia, Serbia person Andrea Pirković University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia doktor bioloških nauka person 0000-0002-1182-4895 Antonije Onjia University of Belgrade, Faculty of Technology and Metallurgy, Serbia person Aleksandar Marinković University of Belgrade, Faculty of Technology and Metallurgy, Serbia person Aleksandra A. Jovanović University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia doktor tehnoloških nauka person 0000-0001-5394-0125 application/pdf 474910 https://phaidrabg.bg.ac.rs/o:34253 no yes 19 70 92000004 11A24 11A29 11A41 Antioxidant capacity of silibinin-loaded liposomes 75 Book of Abstracts 3rd UNIFood International Conference – UNIFood2024 Belgrade, Serbia 2024