o:34252 The influence of lyophilization on liposomal particles with silymarin en Silymarin is the group of biologically active polyphenols from Silybum marianum (milk thistle) that contains silibinin, isosilybin, silydianin, and silychristand. The mentioned components show numerous pharmacological activities promoting human health and well-being, including antioxidant, antimicrobial, anti-inflammatory, antiviral, immunomodulatory, and antitumor effects. Nevertheless, silymarin is quite sensitive to temperature, light, and oxidation and has poor water solubility and low bioavailability. Therefore, their application in food, functional food, dietetic supplements, and pharmaceutics is limited. The encapsulation of silymarin in liposomes represents a technique that can be widely used to strengthen and supplement formulations by enhancing stability and bioavailability and controlling the delivery of the active compound. Lyophilization is a widely employed procedure for drying thermosensitive components to obtain freeze-dried products with active compounds that are stable over a long period, due to the prevention of hydrolytic and oxidative degradation which can occur in water surrounding. Hence, lyophilization can result in significant modifications of the liposomal vesicles, thus its effect should be examined. The liposomes were prepared using 0.5 g of silymarin, 5 g of phospholipids, 10 mL of ethanol, and 40 mL of water in the proliposome procedure. After the preparation, the liposomes were freeze-dried for 24 h. The characterization is performed using photon correlation spectroscopy. Vesicle size and polydispersity index (PDI) of lyophilized silymarin-loaded liposomes were changed from 4080.0±24.0 nm and 0.346±0.044 to 4628.1±45.2 nm 0.426±0.038, respectively. Zeta potential was -20.55±1.34 mV, mobility was -1.55±0.13 µmcm/Vs, and conductivity was 20.15±1.06 µS/cm. In comparison to non-treated liposomes, lyophilization caused an increase in vesicle size and the absolute value of zeta potential, and a decrease in the conductivity value. On the other hand, freeze drying did not have a significant influence on PDI values and mobility of silymarin-loaded liposomes. lyophilization, silymarin, size, zeta potential 1552100 978-86-7834-438-1 2024-07-10T07:48:54.184Z 44 yes 46 Amjed Karkad University of Belgrade Faculty of Technology and Metallurgy Petar Batinić Institute for Medicinal Plants Research “Dr Josif Pančić”, Belgrade, Serbia person Milena Milošević University of Belgrade, Institute of Chemistry, Technology and Metallurgy, National Institute of the Republic of Serbia, Serbia person Andrea Pirković University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia doktor bioloških nauka person 0000-0002-1182-4895 Antonije Onjia University of Belgrade, Faculty of Technology and Metallurgy, Serbia person Aleksandar Marinković University of Belgrade, Faculty of Technology and Metallurgy, Serbia person Aleksandra A. Jovanović University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia doktor tehnoloških nauka person 0000-0001-5394-0125 application/pdf 474910 https://phaidrabg.bg.ac.rs/o:34252 no yes 19 70 92000004 11A24 11A41 11A29 The influence of lyophilization on liposomal particles with silymarin 76 Book of Abstracts 3rd UNIFood International Conference – UNIFood2024 Belgrade, Serbia 2024