o:34038 Fumaria officinalis L. as a novel source of biologically active compounds en Fumaria officinalis L. (Fumariaceae) is a scrambling annual plant, distributed and cultivated throughout Europe, and represents a component of various phytotherapeutic formulations in the European ethnobotany used in hepatobiliary dysfunction, illnesses of gastrointestinal and urogenital tracts, cancer, rheumatism, high blood pressure, and skin disorders. The study aimed to determine the total polyphenol and flavonoid contents (TPC and TFC, respectively) in F. officinalis extracts prepared using maceration and heat-assisted extraction (HAE), as well as to investigate their cytotoxicity on human keratinocyte cells (HaCaT), antioxidant potential, and spectrophotometric sun protecting factor (SPFs). The influence of extracts on the viability of keratinocytes was investigated in the MTT assay, while antioxidant capacity was tested using ABTS and DPPH assays. SPFs value was calculated using the Mansur equation and the absorbance measurements (290-320 nm) with the aim of expressing the photoprotective activity of the extracts. The polyphenol concentration in lyophilized extracts was 165.5±1.1 mg/g (maceration) and 171.8±1.4 mg/g (HAE), whereas the flavonoid content amounted to 71.6±1.9 mg/g (maceration) and 80.4±1.6 mg/g (HAE). The results show that ABTS and DPPH radical scavenging capacity of lyophilized extracts was 11.5±1.0 mmol Trolox/g and IC50DPPH 0.36±0.03 mg/mL (maceration) and 12.9±0.8 mmol Trolox/g and IC50DPPH 0.28±0.02 mg/mL (HAE). SPFs values for the extracts at the concentration of 100 µg/mL were 1.162±0.005 (maceration) and 1.255±0.015 (HAE). The higher antioxidant potential and SPFs of HAE extracts can be explained by the higher polyphenol and flavonoid contents. In vitro cytotoxicity assay in HaCaT cells showed that both types of extracts at all tested concentrations (25-100 µg/mL) did not reduce the number of viable cells. Additionally, HAE extract significantly increased the number of viable keratinocytes at the concentration of 100 µg/mL. The presented study provides evidence of the polyphenol and flavonoid yields in F. officinalis extracts and their biological/pharmacological potential. Due to its proven antioxidant potential and positive impact on human keratinocytes, HAE extract can add value and/or improve the quality of the existing pharmaceutical and cosmetic products for the skin. 2024-06-17T15:25:54.329Z 44 yes 46 Dragana Dekanski University of Belgrade, Institute for the Application of Nuclear Energy, Serbia doktor medicinskih nauka 0000-0001-8467-4165 Andrea Pirković University of Belgrade, Institute for the Application of Nuclear Energy, Serbia doktor nauka person 0000-0002-1182-4895 Aleksandra Jovanović University of Belgrade, Institute for the Application of Nuclear Energy, Serbia doktor tehnoloških nauka person 0000-0001-5394-0125 application/pdf 325861 https://phaidrabg.bg.ac.rs/o:34038 no yes 19 70 92000004 11A29 11A2910 11A29 11A2901 Fumaria officinalis L. as a novel source of biologically active compounds PP211 Book of Abstracts of 9th European Congress of Pharmacology, EPHAR2024, Athens, Greece 2024