o:33847 Glycosylation and Characterization of Human Transferrin in an End-Stage Kidney Disease Glikozilacija i karakterizacija humanog transferina u terminalnoj bubrežnoj insuficijenciji en ABSTRACT Chronic kidney disease (CKD) is a global health concern affecting approximately one billion individuals worldwide. End-stage kidney disease (ESKD), the most severe form of CKD, is often accompanied by anemia. Peritoneal dialysis (PD), a common treatment for ESKD, utilizes the peritoneum for solute transfer but is associated with complications including protein loss, including transferrin (Tf) a key protein involved in iron transport. This study investigated Tf characteristics in ESKD patients compared to healthy individuals using lectin microarray, spectroscopic techniques and immunocytochemical analysis to assess Tf interaction with transferrin receptors (TfRs). ESKD patients exhibited altered Tf glycosylation patterns, evidenced by significant changes in lectin reactivity compared to healthy controls. However, structural analyses revealed no significant differences in the Tf secondary or tertiary structures between the two groups. A functional analysis demonstrated comparable Tf-TfR interaction in both PD and healthy samples. Despite significant alterations in Tf glycosylation, structural integrity and Tf-TfR interaction remained preserved in PD patients. These findings suggest that while glycosylation changes may influence iron metabolism, they do not impair Tf function. The study highlights the importance of a glucose-free dialysis solutions in managing anemia exacerbation in PD patients with poorly controlled anemia, potentially offering a targeted therapeutic approach to improve patient outcomes. transferrin; glycosylation; end-stage kidney disease; peritoneal dialysis; structure; lectin microarray; FTIR 1552099 10.3390/ijms25094625 2024-05-28T09:22:59.140Z 44 yes 46 Goran Miljuš University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia doktor biohemijskih nauka 0000-0001-6417-8711 Ana Penezić University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia doktor biohemijskih nauka person 0000-0002-2539-4507 Lucia Pažitná Institute of Chemistry, Slovak Academy of Sciences, Bratislava, Slovakia person Nikola Gligorijević University of Belgrade, Institute of Chemistry, Technology and Metallurgy, Department of Chemistry, Belgrade, Serbia person Marko Baralić University of Belgrade, School of Medicine, Serbia person Aleksandra Vilotić University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia doktor bioloških nauka person 0000-0002-0898-5401 Miloš Šunderić University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia doktor biohemijskih nauka person 0000-0002-0940-9481 Dragana Robajac University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia doktor biohemijskih nauka person 0000-0002-6423-4645 Zorana Dobrijević University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia doktor bioloških nauka person 0000-0002-4652-7719 Jaroslav Katrlík Institute of Chemistry, Slovak Academy of Sciences, Bratislava, Slovakia person Olgica Nedić University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia doktor biohemijskih nauka person 0000-0003-2042-0056 application/pdf 2424869 https://phaidrabg.bg.ac.rs/o:33847 no yes 20 70 92000001 11A29 11A2905 11A24 11A32 International Journal of Molecular Sciences 25 MDPI 2024