o:33845 Antioxidative and anti-inflammatory effects of taxifolin in H2O2-induced oxidative stress in HTR-8/SVneo trophoblast cell line Antioksidativni i antiinflamatorni efekti taksifolina na modelu oksidativnog stresa indukovanog sa H2O2 u HTR-8/SVneo ćelijskoj liniji trofoblasta en ABSTRACT Oxidative stress has been implicated in numerous pregnancy-related disorders. Biologically active plant secondary metabolites, which are present in everyday diet, could prove effective therapeutic agents in preventing these disorders. This study evaluated effects of taxifolin (dihydroquercetin) on ROS production, markers of oxidative damage to lipids and proteins, activity of antioxidant enzymes and production of pro-inflammatory cytokines in H2O2-induced oxidative stress in trophoblast HTR-8/SVneo cells. Taxifolin in 10 µM and 100 µM concentrations attenuated oxidative damage to lipids and proteins, as evidenced by a decrease in MDA content, extracellular LDH activity, carbonyl groups and nitrite contents. A reduction in the activity of antioxidant enzymes SOD, CAT and GPx in cells pre-treated with taxifolin, prior to H2O2 exposure, was also observed, along with a reduction in intracellular ROS production. Both evaluated concentrations of taxifolin showed anti-inflammatory activity in trophoblast cells, by reducing production of pro-inflammatory cytokines IL-1β and IL-6. In this model of H2O2-induced oxidative stress, taxifolin showed marked antioxidative and anti-inflammatory activities in trophoblast cells, adding further evidence of its protective effects and showing potential as a therapeutic agent in preventing adverse pregnancy outcomes. Taxifolin, Trophoblast, Oxidative stress, Inflammation 1552099 10.1016/j.reprotox.2024.108585 2024-05-28T08:36:49.111Z 44 yes 46 Marija Bruić University of Belgrade, Faculty of Pharmacy, Department of Pathobiology, Belgrade, Serbia Andrea Pirković University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia doktor bioloških nauka person 0000-0002-1182-4895 Sunčica Borozan University of Belgrade, Faculty of Veterinary Medicine, Department of Chemistry, Serbia person Mirjana Nacka Aleksić University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia doktor medicinskih nauka-farmacija person 0000-0003-0750-9347 Milica Jovanović Krivokuća University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia doktor bioloških nauka person 0000-0001-8292-4853 Biljana Spremo-Potparević University of Belgrade, Faculty of Pharmacy, Department of Pathobiology, Belgrade, Serbia person application/pdf 61508 https://phaidrabg.bg.ac.rs/o:33845 no yes 16 70 92000001 11A13 11A29 11A2901 11A10 Reproductive Toxicology 126 Elsevier 2024