o:33456 Characterization of Vaccinium myrtillus leaf extract-loaded liposomes en INTRODUCTION: Vaccinium myrtillus L. leaves contain bioactive components, such as polyphenols, stilbenes, iridoid glycosides, fatty acids, and fibers [1]. However, polyphenols possess low solubility, stability, and bioavailability, thus the encapsulation of the mentioned active principles in different carriers is necessary [2]. Liposomes are widely used as a carrier for the encapsulation, preservation, and controlled release of polyphenols in various products [3]. Therefore, the aims of the presented research are the development and characterization of V. myrillus leaf extract-loaded liposomes via the determination of encapsulation efficiency, particle size, polydispersity index (PDI), zeta potential, and mobility. EXPERIMENTAL: The liposomes with encapsulated extract were prepared employing the proliposome procedure [3]. Encapsulation efficiency was indirectly calculated by the polyphenol concentration determined in the supernatant. Particle size, PDI, zeta potential, and mobility were measured by the photon correlation spectroscopy in Zetasizer. Every measurement was examined three times at 25°C. RESULTS AND DISCUSSION: The encapsulation efficiency of polyphenols was >85%. The liposomes contained only phospholipids resulting in a more rigid membrane [4] providing the prevention of the leakage of the encapsulated polyphenols, as well as a higher encapsulation efficiency. The diameter and PDI of the liposomes were 5408.7±56.4 and 0.249±0.049 nm, respectively confirming that higher liposomal vesicles possessed lower PDI values [4]. The zeta potential and mobility were -5.02±0.25 mV and -0.315±0.016 μmcm/Vs, respectively. Zeta potential possessed negative values that are related to the exposure of the phosphate group lying in an outer plane concerning the choline groups [4]. The mobility of liposomes represents a function of the size, zeta potential, and lipid composition. The liposomal vesicles with lower membrane fluidity also show low mobility. The changes in the mobility of the liposomes were attributed to the membrane fluidity and ability to deform. Additionally, when flavonoids (also presented in V. myrtillus extract) are adsorbed at the surface of the liposomes, it can decrease their mobility [5]. CONCLUSIONS: The beneficial effects of bioactive principles from V. myrtillus leaves on human health and their sensitivity highlight the application of liposomal particles as a carrier for V. myrtillus extract and their potential implementation in foods, functional foods, pharmaceutics, and cosmetics. 1552101 0367–598X 2024-04-15T13:45:15.525Z 44 yes 46 Muna Elferjane University of Misurata, Faculty of Nursing and Health Sciences, Misurata, Libya Milena Milosevic Institute of Chemistry, Technology and Metallurgy, University of Belgrade, Belgrade, Serbia person Vojislav Ciric Faculty of Medicine, University of Niš, Niš, Serbia person Petar Batinić Institute for Medicinal Plants Research “Dr Josif Pančić” Belgrade, Serbia person Branko Bugarski University of Belgrade, Faculty of Technology and Metallurgy, Serbia person Aleksandar Marinković University of Belgrade, Faculty of Technology and Metallurgy, Serbia person Aleksandra A. Jovanović University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia doktor tehnoloških nauka person 0000-0001-5394-0125 application/pdf 2376280 https://phaidrabg.bg.ac.rs/o:33456 no yes 1 70 92000004 11A41 11A24 11A29 Characterization of Vaccinium myrtillus leaf extract-loaded liposomes 1s 65 ExcellMater Conference 2024: Innovative Biomaterials for Novel Medical Devices 2024-04-10