o:32858 Serum Glycome as a Diagnostic and Prognostic Factor in Gestational Diabetes Mellitus Serumski glikom kao dijagnostički i prognostički marker gestacijskog dijabetesa en ABSTRACT Gestational diabetes mellitus (GDM) is a risk factor for both mother and fetus/neonate during and after the pregnancy. Inconsistent protocols and cumbersome screening procedures warrant the search for new and easily accessible biomarkers. We investigated a potential of serum N-glycome to differentiate between healthy pregnant women (n = 49) and women with GDM (n = 53) using a lectin-based microarray and studied the correlation between the obtained data and parameters of glucose and lipid metabolism. Four out of 15 lectins used were able to detect the differences between the control and GDM groups in fucosylation, terminal galactose/N-acetylglucosamine (Gal/GlcNAc), presence of Galα1,4Galβ1,4Glc (Gb3 antigen), and terminal α2,3-sialylation with AUC values above 60%. An increase in the Gb3 antigen and α2,3-sialylation correlated positively with GDM, whereas the amount of fucosylated glycans correlated negatively with the content of terminal Gal/GlcNAc. The content of GlcNAc oligomers correlated with the highest number of blood analytes, indices, and demographic characteristics, but failed to discriminate between the groups. The presence of terminal Gal residues correlated positively with the glucose levels and negatively with the LDL levels in the non-GDM group only. The results suggest fucosylation, terminal galactosylation, and the presence of Gb3 antigen as prediction markers of GDM. OGTT, GDM, glycosylation, microarray, lectins, Shiga holotoxin B subunit lectin 1552099 10.1134/S0006297924010097 1552101 0006-2979 2024-02-24T20:01:37.273Z 44 yes 46 Ognjen Radojičić Department of High-Risk Pregnancies, University Clinic for Gynecology and Obstetrics “Narodni Front”, Belgrade, Serbia Dragana Robajac University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia doktor biohemijskih nauka person 0000-0002-6423-4645 Olgica Nedić University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia doktor biohemijskih nauka person 0000-0003-2042-0056 Željko Miković Medical School, University of Belgrade, Belgrade, Serbia person Vesna Mandić Marković Medical School, University of Belgrade, Belgrade, Serbia person Jaroslav Katrlik Institute of Chemistry, Slovak Academy of Sciences, Bratislava, Slovakia person Kristina Kianičkova Institute of Chemistry, Slovak Academy of Sciences, Bratislava, Slovakia person Paras Kundalia Institute of Chemistry, Slovak Academy of Sciences, Bratislava, Slovakia person Zorana Dobrijević University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia doktor bioloških nauka person 0000-0002-4652-7719 Lucia Pažitna Institute of Chemistry, Slovak Academy of Sciences, Bratislava, Slovakia person application/pdf 59519 https://phaidrabg.bg.ac.rs/o:32858 no yes 18 70 92000001 11A29 11A2905 11A32 Biochemistry (Moscow) 89 1 148 158 Pleiades Publishing, Ltd. 2024