o:32706 Detecting thyrotropin receptor mRNA from peripheral blood of patients with differentiated thyroid cancer rules out non-aggressive cases Detekcija iRNK receptora tirotropina iz periferne krvi pacijenata sa diferenciranim karcinomom štitne žlezde isključuje neagresivne slučajeve en ABSTRACT Early diagnosis of thyroid cancer is hampered by the inability of fine-needle aspiration biopsy (FNAB) to accurately classify ∼30% of cases while preoperative cancer staging detects lymph nodal involvement in only half of cases. Liquid biopsy may present an accurate, non-invasive alternative for preoperative thyroid nodule assessment. Thyrotropin receptor (TSHR) mRNA, a surrogate marker for circulating cancer cells (CTC), may be an option for early detection of malignancy from peripheral blood, but requires methodological improvements. We aimed to investigate if TSHR mRNA can be detected in low sample volumes by employing an ultrasensitive method-droplet digital PCR (ddPCR). Circulating tumour cells, droplet digital PCR, liquid biopsy, thyroid cancer, thyrotropin receptor mRNA 1552099 10.1177/00045632241228217 2024-02-06T08:39:22.126Z 44 yes 46 Jelena Janković Miljuš University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia doktor biohemijskih nauka 0000-0002-2589-3306 Uršula Prosenc Zmrzljak Molecular Biology Laboratory, BIA Separations CRO-Labena d.o.o, Ljubljana, Slovenia person Rok Košir Molecular Biology Laboratory, BIA Separations CRO-Labena d.o.o, Ljubljana, Slovenia person Milan Jovanović University Clinical Center of Serbia, Center for Endocrine Surgery, Belgrade, Serbia person Ilona Đorić University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia doktor bioloških nauka person 0000-0001-5322-4559 Jelena Rončević University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia person 0000-0003-3656-6420 Tijana Išić Denčić University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia doktor bioloških nauka person 0000-0001-8475-2266 Sonja Šelemetjev University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia doktor bioloških nauka person 0000-0002-2898-6671 application/pdf 127487 https://phaidrabg.bg.ac.rs/o:32706 no yes 18 70 92000001 11A29 11A2902 Annals of Clinical Biochemistry Sage Journals 2024