Detecting thyrotropin receptor mRNA from peripheral blood of patients with differentiated thyroid cancer rules out non-aggressive cases eng https://phaidrabg.bg.ac.rs/o:32706 doi:10.1177/00045632241228217 http://creativecommons.org/licenses/by-nc-nd/4.0/legalcode info:eu-repo/semantics/article ABSTRACT Early diagnosis of thyroid cancer is hampered by the inability of fine-needle aspiration biopsy (FNAB) to accurately classify ∼30% of cases while preoperative cancer staging detects lymph nodal involvement in only half of cases. Liquid biopsy may present an accurate, non-invasive alternative for preoperative thyroid nodule assessment. Thyrotropin receptor (TSHR) mRNA, a surrogate marker for circulating cancer cells (CTC), may be an option for early detection of malignancy from peripheral blood, but requires methodological improvements. We aimed to investigate if TSHR mRNA can be detected in low sample volumes by employing an ultrasensitive method-droplet digital PCR (ddPCR). Sage Journals Circulating tumour cells, droplet digital PCR, liquid biopsy, thyroid cancer, thyrotropin receptor mRNA Annals of Clinical Biochemistry application/pdf 127487 bytes Janković Miljuš, Jelena Prosenc Zmrzljak, Uršula Košir, Rok Jovanović, Milan Đorić, Ilona Rončević, Jelena Išić Denčić, Tijana Šelemetjev, Sonja 2024