o:32253 Antioxidant, antibacterial and enzyme inhibitory activity of the leaf extracts of Paeonia daurica Andrews wild growing in Serbia en This study was designed to investigate the impact of different extraction methods (ultrasound-assisted extraction, maceration, and microwave-assisted extraction) on the content of polyphenols and biological activities of leaf extract of Paeonia daurica subsp. daurica collected in Južni Kucaj (Serbia). The total polyphenol and flavonoid content were achieved by the UV-Vis spectroscopy. FTIR analysis was conducted to investigate structural properties of the extracts. In vitro methods (2,2′ -azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) - ABTS and 2,2-diphenyl-1-picrylhydrazyl – DPPH assays) were carried out to measure the antioxidant activity of the extracts, while their antibacterial activity was tested against three Gram-positive and three Gram-negative bacterial strains. Analyzing the results of antioxidant activity, the highest anti-DPPH potential was achieved in the extracts of LPD obtained by maceration. The extraction method proven to produce the most effective antibacterial agents is maceration. Moreover, inhibitory activity of extracts against acetyl–and butyrylcholinesterase, α-amylase, α-glucosidase, and tyrosinase was evaluated in the enzyme inhibition assays. Overall, the results of this research indicated the possible use utilization of leaf extracts of Paeonia daurica subsp. daurica Andrews in food technology and pharmacy. P. daurica subsp.daurica Andrews; total polyphenol content; structural analysis; biological activities 1552099 s https://doi.org/10.61652/leksir2343e159B 2024-01-09T13:02:44.851Z 44 yes 46 Petar Batinić Institute for Medicinal Plants Research “Dr Josif Pančić”, Belgrade, Serbia Natalija Cutovic Institute for Medicinal Plants Research “Dr Josif Pančić”, Belgrade, Serbia person Aleksandra Jovanović University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia doktor nauka person 0000-0001-5394-0125 Dejan Stojković University of Belgrade, Institute for Biological Research "Siniša Stankovic", National Institute of the Republic of Serbia person Gokhan Zenging Selcuk University, Science Faculty, Konya, Turkey person Tamara Carevic University of Belgrade, Institute for Biological Research "Siniša Stankovic", National Institute of the Republic of Serbia person Zeljana Prijić Institute for Medicinal Plants Research “Dr Josif Pančić”, Belgrade, Serbia person Aleksandar Marinkovic University of Belgrade, Faculty of Technology and Metallurgy, Belgrade, Serbia person Tatjana Markovic Institute for Medicinal Plants Research “Dr Josif Pančić”, Belgrade, Serbia person application/pdf 3869730 https://phaidrabg.bg.ac.rs/o:32253 no yes 19 70 1552253 11A21 11A29 11A41 Antioxidant, antibacterial and enzyme inhibitory activity of the leaf extracts of Paeonia daurica Andrews wild growing in Serbia e159 43 Lekovite sirovine 2023-12-25