o:32086 BRAFV600E, BANCR, miR-203a-3p and miR-204-3p in Risk Stratification of PTC Patients BRAFV600E, BANCR, miR-203a-3p i miR-204-3p u stratifikaciji rizika pacijenata obolelih od papilarnog karcinoma štitaste žlezde en ABSTRACT In order to enhance the risk stratification of papillary thyroid carcinoma (PTC) patients, we assessed the presence of the most common mutation in PTC (BRAFV600E) with the expression profiles of long non-coding RNA activated by BRAFV600E (BANCR) and microRNAs, which share complementarity with BANCR (miR-203a-3p and miR-204-3p), and thereafter correlated it with several clinicopathological features of PTC. BRAFV600E was detected by mutant allele-specific PCR amplification. BANCR and miRs levels were determined by quantitative RT-PCR. Bioinformatic analysis was applied to determine the miRs’ targets. The expression profile of miR-203a-3p/204-3p in PTC was not affected by BRAFV600E. In the BRAFV600E-positive PTC, high expression of miR-203a-3p correlated with extrathyroidal invasion (Ei), but the patients with both high miR- 203a-3p and upregulated BANCR were not at risk of Ei. In the BRAFV600E-negative PTC, low expression of miR-204-3p correlated with Ei, intraglandular dissemination and pT status (p < 0.05), and the mutual presence of low miR-204-3p and upregulated BANCR increased the occurrence of Ei. Bioinformatic analysis predicted complementary binding between miR-203a-3p/204-3p and BANCR. The co-occurrence of tested factors might influence the spreading of PTC. These findings partially describe the complicated network of interactions that may occur during the development of PTC aggressiveness, potentially providing a new approach for high-risk PTC patient selection. papillary thyroid carcinoma; long non-coding RNA; microRNAs; tumor markers; aggressiveness; prognosis 1552099 10.3390/biomedicines11123338 2023-12-19T12:11:04.892Z 44 yes 46 Stefana Stojanović University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia 0000-0003-3808-0467 Sonja Šelemetjev University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia doktor bioloških nauka person 0000-0002-2898-6671 Ilona Đorić University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia doktor bioloških nauka person 0000-0001-5322-4559 Jelena Janković Miljuš University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia doktor biohemijskih nauka person 0000-0002-2589-3306 Svetislav Tatić University of Belgrade, Faculty of Medicine, Institute for Pathology, Serbia person Vladan Živaljević University Clinical Center of Serbia, Clinic for Endocrine Surgery, Belgrade, Serbia person Tijana Išić Denčić University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia doktor bioloških nauka person 0000-0001-8475-2266 application/pdf 1417265 https://phaidrabg.bg.ac.rs/o:32086 no yes 16 70 92000001 11A29 11A2902 11A32 11A3240 Biomedicines 11 12 MDPI 2023