o:31710 Comparison of maceration and ultrasound-assisted extraction of antioxidant compounds from Vaccinium myrtillus L. en ABSTRACT Vaccinium myrtillus L.(Ericaceae), a perennial, wild, and small deciduous shrub that grows in the mountains and forests of Europe, contains anthocyanins, phenolic acids, flavonoids, fatty acids, stilbenes, iridoid glycosides, dietary fibers, vitamins, and minerals. The leaves' extracts are widely used in traditional medicine due to their astringent, antiseptic, antioxidant, anti-inflammatory, skin-rejuvenating, lipid-lowering, hypolipidemic, and hypoglycemic activities. The novel extraction techniques, including ultrasound-assisted extraction, provide various benefits, such as reducing solvent consumption and extraction time and increasing extraction yield, and quality. Hence, in the present study, V. myrtillus extracts were prepared using dried leaves (0.66 g), 50% ethanol as the extraction solvent (20 mL), and maceration (60 min) or ultrasound-assisted extraction (ultrasound probe, amplitude of 60% for 5 min). The obtained extracts were examined in terms of total polyphenol content (TPC) and antioxidant activity (ABTS, DPPH, FRAP, and CUPRAC assays). The TPC of the extract prepared using maceration was 55.2±0.7 mg gallic acid equivalents (GAE)/g of plant material, while the TPC of the extract prepared using an ultrasound probe was 55.6±1.0 mg GAE/g. The DPPH radical scavenging activities of the extracts correlated with the TPC and amounted to 1.81±0.05 mg/mL for macerate and 1.79±0.02 mg/mL for the extract obtained using an ultrasound probe, whereas ABTS antioxidant capacity did not correlate with the polyphenol concentration, 31.4±0.9 µmol Trolox equivalents (TE)/g for the macerate and 43.4±1.1 µmol TE/g for the extract from the ultrasound-assisted extraction. According to the results of FRAP and CUPRAC assays, the antioxidant potential was similar for both extracts (15.3±0.2 and 15.5±0.2 µmol Fe2+/g and 45.7±0.5 and 46.0±0.7 µmol TE/g, respectively). Due to higher ABTS radical scavenging potential and significantly shorter extraction time, V. myrtillus extract prepared using an ultrasound probe was favored. The present research was an initial step in the preparation of V. myrtillus extracts which can be potentially implemented in food, pharmaceutical, and cosmetic formulations. Antioxidant activity, maceration, ultrasound-assisted extraction, polyphenols, Vaccinium myrtillus UDK 633.88:66.061 2023-11-20T08:41:43.461Z 44 yes 46 Petar Batinić Institute for Medicinal Plants Research "Dr Josif Pančić", Belgrade, Serbia Aleksandra A. Jovanović University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia doktor tehnoloških nauka person 0000-0001-5394-0125 Muna Rajab Elferjane University of Misurata, Faculty of Nursing and Health Sciences, Misurata, Libya person Natalija Čutović Institute for Medicinal Plants Research "Dr Josif Pančić", Belgrade, Serbia person Milena Milošević University of Belgrade, Institute of Chemistry, Technology and Metallurgy, Serbia person Aleksandar Marinković University of Belgrade, Faculty of Technology and Metallurgy, Serbia person Branko Bugarski University of Belgrade, Faculty of Technology and Metallurgy, Serbia person application/pdf 387142 https://phaidrabg.bg.ac.rs/o:31710 no yes 18 70 92000004 11A29 11A2910 11A24 11A41 VIII International Congress "Engineering, Environment and Materials in Process Industry", Jahorina, Bosnia and Herzegovina 2023