o:31670 In vitro antioxidant activity of cotton fabric treated wit ethanol and water Thymus serpyllum L. (wild thyme) extracts en ABSTRACT The plants contain a large number of various compounds that exhibit significant antioxidant activity. Among them, polyphenols and flavonoids are the most studied compounds with different biological properties, including antioxidant activity. The functionalization of cotton fabric with natural compounds from plants results in the material with improved antioxidative properties. In this study, cotton fabrics treated with ethanol and water Thymus serpyllum L. (wild thyme) extracts were investigated in terms of antioxidant properties and chemical composition. In vitro antioxidant capacity of ethanol and water extracts, and the cotton fabric before and after functionalization was evaluated using 2,2-diphenyl-1-picrylhydrazyl (DPPH•), and 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid (ABTS•+) radicals scavenging capacity assays. FTIR and UV-Vis spectroscopy elucidated the structure of the cotton fabric, extracts, and functionalized cotton fabric. FTIR spectroscopy demonstrated that the structure of cellulose was dominated in cotton, and also proved the presence of a low percentage of protein, pectin, oil, fat, and wax, as well as, binding of compounds from the extract by cross-linking with long chains of structure cotton. In dry extracts, the presence of various chemical functional groups was confirmed. UV-Vis analysis identified the active components in T. serpyllum extracts. Namely, absorption values for both spectra (water and ethanol samples) at 215-253 nm, 263-384 nm, are specific for phenolic acids, and flavonoids. Also, one of the aims of the study was to investigate the influence of various extracts (water and ethanol samples) on the antioxidant activity of cotton fabric. All extracts demonstrated excellent antioxidant properties, more potent than the reference antioxidant (ascorbic acid) in both antioxidant assays (between 85-86% in DPPH and 94-96% in ABTS assays). Pure T. serpyllum water extract was the most potent in the DPPH assay (86%), while pure ethanolic extract was the most potent antioxidant in the ABTS assay (96%). The antioxidant capacity of raw cotton fabric was insufficient (4%). On the other hand, in the DPPH assay, samples of cotton fabric with extract showed moderate activity with scavenging activity of 48% and 45% for ethanol and water extracts, respectively. Additionally, in the ABTS assay, the antioxidant activity of treated cotton fabric was 51% and 46% for ethanol and water extracts, respectively. Thymus serpyllum extract, cotton knitted fabric, antioxidant activity 1552100 978-99955-81-45-9 2023-11-14T13:54:42.609Z 44 yes 46 Milena Milošević University of Belgrade, Institute of Chemistry, Technology and Metallurgy, Serbia Aleksandra A. Jovanović University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia doktor tehnoloških nauka person 0000-0001-5394-0125 Petar Batinić Institute for Medicinal Plants Research "Dr Josif Pančić", Serbia person Dragana Grujić University of Banja Luka, Faculty of Technology, Bosnia and Herzegovina person Nataša Knežević University of Belgrade, Department of Chemical Dynamics and Permanent Education, "VINČA", Institute of Nuclear Sciences-National Institute of the Republic of Serbia, Serbia person Aleksandar Marinković University of Belgrade, Faculty of Technology and Metallurgy, Serbia person Jovana Milanović Innovation centre of the Faculty of Technology and Metallurgy, Serbia person application/pdf 581860 https://phaidrabg.bg.ac.rs/o:31670 no yes 18 70 92000004 11A24 11A29 11A28 11A41 Proceedings-VIII International Congress-Engineering, Environment and Materials in Process Industry, EEM 2023 University of East Sarajevo, Faculty of Technology 2023