o:31387 Stimulation and inhibition of NF-B by repurposed drugs – effects on hamster fibrosarcoma en Abstract: Background. NF-B transcription factors are key regulators of apoptosis, autophagy, necroptosis and turns up everywhere in cancer life and death. This study investigated how the regulation of NF-B by repurposed drugs in oncology affect experimental fibrosarcoma development and progression in hamsters. Material and Methods. Anticancer efficacy of certain drugs was tested on fibrosarcoma experimentally induced by BHK21/C13 cells in Syrian golden hamsters. Used repurposed drugs with in vitro verified NF-B inhibitory effect were: metformin, caffeine, itraconazole, nitroglycerin. Used drug with known NF-B stimulatory effect was mebendazole. Tumor biophysical characteristics, histology and immunohistochemistry were assessed. Blood samples were collected for hematological and biochemical analyses and the main organs were toxicologically analyzed. Results. Our study showed that combinations of NF-B inhibitors: metformin with caffeine, metformin with itraconazole and metformin with nitroglycerin, in human equivalent doses could be efficaceous (P < 0.05) against fibrosarcoma growth, which can rescued by mebendazole, without toxicity and influence on biochemical and hematological tests. Conclusion. Combinations of repurposed drugs with NF-B inhibitory effect: metformin with caffeine, metformin with itraconazole and metformin with nitroglycerin could be an important therapeutic option in oncology. Keywords : BHK-21/C13 cell culture, drug effects, fibrosarcoma, hamsters, NF-kB 1552101 3009-3848 1552102 https://www.sdir.ac.rs/pdf/Oncology_Insights_No_1_2023.pdf 2023-10-23T15:01:35.338Z 44 no 46 Kosta Popović J. 0000-0001-7895-9009 Dušica Popović J. Državni univerzitet u Novom Pazaru person 0000-0001-7880-0874 Dejan Miljković person 0000-0002-7739-5202 Dušan Lalošević person 0000-0002-0760-9180 Zana Dolićanin Državni univerzitet u Novom Pazaru person 2237799 0000-0003-0457-3087 Ivan Čapo person 0000-0001-7531-5099 Jovan Popović K. person 0000-0002-6790-8668 application/pdf 1113379 https://phaidrabg.bg.ac.rs/o:31387 no yes 1 70 92000004 20A01 Proceedings Book of the 6th Congress of the Serbian Association for Cancer Research – SDIR with international participation “From Collaboration to Innovation in Cancer Research”, October 2-4 2023, Belgrade, Serbia. Official Journal of the Serbian Association for Cancer Research Oncology Insights, October 2023 1 1 101 101 2023