o:31072 Fibrinogen glycosylation of end-stage renal disease patients on peritoneal dialysis en ABSTRACT Glycosylation may have significant impact on structure and function of a certain protein. Patients with end-stage renal disease (ESRD) are at high risk for developing cardiovascular complications with their haemostasis being characterised with delayed fibrin clot formation, increased clot strength, and delayed cloth lysis. Since fibrinogen is the key protein in the processes of fibrin clot formation and lysis, we isolated this protein from the plasma of patients with ESRD on peritoneal dialysis (ESRD-PD) and analysed glycosylation of the native whole fibrinogen and its individual chains by lectin-based microarray and lectin blotting. Compared to healthy controls, fibrinogen from ESRD-PD patients had increased levels of bisecting biantennary bigalactosylated (A2BG2) glycan and decreased levels of core-fucosylated biantennary (FA2) glycan. The distribution of glycans on individual chains was also altered, with the γ-chain being the most affected. Increased levels of multi-antennary N-glycans in ESRD-PD patients were also associated with the type of dialysis solutions, whereas an increase in the fucosylation levels was strongly related to the peritoneal membrane damage. Investigation of fibrinogen glycosylation can offer better insight into fibrinogen-related complications observed in ESRD-PD patients and, additionally, contribute to prognosis, choice of personalised therapy, determination of peritoneal membrane damage, and the length of the utilization of peritoneum for dialysis. glycan; fibrinogen; lectin; microarray; peritoneal dialysis 2023-10-07T19:12:50.827Z 44 yes 46 Marko Baralić Department of Nephrology, Clinical Centre of Serbia, Belgrade, Serbia Nikola Gligorijević University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia person Voin Brković Department of Nephrology, Clinical Centre of Serbia, Belgrade, Serbia person Jaroslav Katrlík Institute of Chemistry, Slovak Academy of Sciences, Bratislava, Slovakia person Lucia Pažitná Institute of Chemistry, Slovak Academy of Sciences, Bratislava, Slovakia person Miloš Šunderić University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia doktor biohemijskih nauka person 0000-0002-0940-9481 Goran Miljuš University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia doktor biohemijskih nauka person 0000-0001-6417-8711 Ana Penezić University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia doktor biohemijskih nauka person 0000-0002-2539-4507 Zorana Dobrijević University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia doktor bioloških nauka person 0000-0002-4652-7719 Mirjana Laušević School of Medicine, University of Belgrade, Serbia person Olgica Nedić University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia doktor biohemijskih nauka person 0000-0003-2042-0056 Dragana Robajac University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia doktor biohemijskih nauka person 0000-0002-6423-4645 application/pdf 181002 https://phaidrabg.bg.ac.rs/o:31072 no yes 18 70 92000004 11A29 11A2905 11A32 International Fibrinogen Research Society-Mini-symposium 50 International Fibrinogen Research Society, Université de Genève, Faculté de Médecine 2021