o:30964 Optimization of the extraction process from Satureja montana L. physicochemical characterization of the extracts Optimizacija procesa ekstrakcije iz biljke Satureja montana l.: fizičkohemijska karakterizacija ekstrakata en ABSTRACT The presented study aimed to optimize polyphenol extraction from Satureja montana L. obtained from the experimental field of the Institute for Medicinal Plants Research ”Dr Josif Pančić”, Serbia, by varying the most important parameters for maceration, solid-to-solvent ratio, and extraction time. The obtained extracts were characterized regarding the total polyphenol content (TPC), total flavonoid content (TFC), antioxidant potential, extraction yield, conductivity, density, surface tension, and viscosity. The TPC and TFC were 7 to 92 mg GAE (gallic acid equivalent)/g and 3.7 to 10.9 mg CE (catechin equivalent)/g, respectively. The highest extraction yield (86 %) as well as the highest antioxidant activities were obtained for the extracts prepared using a solid-to-solvent ratio of 1 g :50 cm3. On the other hand, the extraction time did not have a significant influence. The highest conductivity was measured in the extract prepared at a 1 g :10 cm3 ratio, while the highest density and surface tension were in the extract prepared at a 1 g :40 cm3 ratio (941 kg m-3 and 29.0 mN m-1, respectively). The highest viscosity was measured in the extract prepared at a 1 g : 20 cm3 ratio (2.89 mPa∙s). Our study shows the possibilities for the production of polyphenol-rich extract of S. montana which might be used in pharmaceutical, food, or cosmetic products. antioxidant activity; flavonoids; isolation; polyphenols; winter savory 1552099 10.2298/HEMIND221213020C 2023-10-02T09:48:31.569Z 44 yes 46 Natalija Čutović Institute for Medicinal Plants Research “Dr Josif Pančić”, Belgrade, Serbia Petar Batinić Institute for Medicinal Plants Research “Dr Josif Pančić”, Belgrade, Serbia person Tatjana Marković Institute for Medicinal Plants Research “Dr Josif Pančić”, Belgrade, Serbia person Dragoja Radanović Institute for Medicinal Plants Research “Dr Josif Pančić”, Belgrade, Serbia person Aleksandar Marinković University of Belgrade, Faculty of Technology and Metallurgy, Serbia person Branko Bugarski University of Belgrade, Faculty of Technology and Metallurgy, Serbia person Aleksandra A. Jovanović University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia doktor tehnoloških nauka person 0000-0001-5394-0125 application/pdf 504171 https://phaidrabg.bg.ac.rs/o:30964 no yes 18 70 92000001 11A41 11A4115 11A29 Optimization of the extraction process from Satureja montana L.: physicochemical characterization of the extracts Association of Chemical Engineers of Serbia 2023