o:30919 Metformin synergized anticancer effect of other repurposed drugs in hamster fibrosarcoma en Abstract: The tumor growth rate positively correlates with glucose levels. The Warburg effect promotes the glucose uptake of cancer cells. The cancer glucose starvation is an effective way for suppressing tumor growth. Reduction of blood glucose can inhibit the proliferation of cancer cells. In addition, the combination of glucose inhibitors could be a promising direction for cancer therapy. Anticancer efficacy of the glucose lowering antidiabetic drug metformin in combinations with 2-Deoxy-D-glucose, deoxycholic acid, caffeine, itraconazole, nitroglycerin and disulfiram was tested on fibrosarcoma experimentally induced by BHK21/C13 cells in Syrian golden hamsters. Tumor biophysical characteristics, histology and immunohistochemistry were assessed. Blood samples were collected for hematological and biochemical analyses and the main organs were toxicologically analyzed. The Combination Index (CI) analysis was used to determine synergistic (CI<1), additive (CI=1) or antagonistic (CI>1) metformin interaction with other investigated drugs. Two-drug combinations with metformin showed significant synergistic (CI<1) antitumor effects on hamster fibrosarcoma compared to control, regarding all tested tumor parameters (P < 0.05) without toxicity. Combinations of metformin with other repurposed drugs might be an effective and safe approach in novel nontoxic adjuvant anticancer treatment. 1552100 ISBN 978-86-7220-140-6 (FOC) 2023-09-26T10:17:07.068Z 44 no 46 Dušica J. Popović Državni univerzitet u Novom Pazaru 0000-0001-7880-0874 Dejan Miljković Faculty of Medicine University Novi Sad person 0000-0002-7739-5202 Dušan Lalošević Faculty of Medicine of Novi Sad person 0000-0002-0760-9180 Zana Dolićanin Državni univerzitet u Novom Pazaru person 2237799 0000-0003-0457-3087 Mihalj Poša Faculty of Medicine, University of Novi Sad person 0000-0002-8044-2655 Ivan Čapo Faculty of Medicine University Novi Sad person 0000-0001-7531-5099 Jovan K. Popović Faculty of Medicine University Novi Sad person 0000-0002-6790-8668 Kosta J. Popović Faculty of Medicine, University of Novi Sad person 0000-0001-7895-9009 application/pdf 237081 https://phaidrabg.bg.ac.rs/o:30919 no yes 1 70 92000004 20A01 Serbian Biochemical Society Twelfth Conference, International scientific meeting "Biochemistry in Biotechnology" 21-23.09.2023. Belgrade, Serbia. 12 67 67 Twelfth Conference, International scientific meeting "Biochemistry in Biotechnology" 21-23.09.2023. Belgrade, Serbia. 2023