o:30823 Paeonia peregrina Mill Petals as a New Source of Biologically Active Compounds: Chemical Characterization and Skin Regeneration Effects of the Extracts en ABSTRACT Paeonia peregrina Mill. is a perennial herbaceous plant species, known for the medicinal value of all of its plant parts, although the chemical composition of the petals is unknown. This study aimed to determine the chemical fingerprint of the petals and also establish the optimal extraction parameters, extraction medium, and extraction method for petals collected from different localities in Serbia. The optimization was performed in order to acquire extracts that are rich in the contents of total polyphenol content (TPC) and total flavonoid content (TFC), and also exhibit strong antioxidant activity. In addition, the influence of the extracts on several human skin pathogens was evaluated, as well as their ability to aid wound closure and act as anti-inflammatory agents. Both the extraction medium and the applied technique significantly influenced the skin-beneficial biological activities, while methanol proved to be a more favorable extraction medium. In conclusion, the extraction conditions that yielded the extract with the richest phenolic content with satisfactory biological potential varied between the assays, while the most promising locality in Serbia for the collection of P. peregrina petals was Pančevo (South Banat). petals; herbaceous peony; biological activities; chemical composition 1552099 10.3390/ijms241411764 2023-09-15T10:30:56.953Z 44 yes 46 Tatjana Marković Institute for Medicinal Plants Research “Dr Josif Pančić”, Belgrade, Serbia Natalija Čutović Institute for Medicinal Plants Research “Dr Josif Pančić”, Belgrade, Serbia person Tamara Carević Department of Plant Physiology, Institute for Biological Research “Siniša Stanković”-National Institute of Republic of Serbia, University of Belgrade, Serbia person Uroš Gašić Department of Plant Physiology, Institute for Biological Research “Siniša Stanković”-National Institute of Republic of Serbia, University of Belgrade, Serbia person Dejan Stojković Department of Plant Physiology, Institute for Biological Research “Siniša Stanković”-National Institute of Republic of Serbia, University of Belgrade, Serbia person Jingqi Xue Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Ministry Agriculture and Rural Affairs, Beijing, China person Aleksandra Jovanović University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia doktor nauka-tehnološko inženjerstvo-biotehnologija person 0000-0001-5394-0125 application/pdf 11287115 https://phaidrabg.bg.ac.rs/o:30823 no yes 16 70 92000001 11A21 11A29 International Journal of Molecular Sciences 24 MDPI 2023