o:30810 Dietary fatty acids as a new binding partner of C-phycocyanin: a fluorimetric study Masne kiseline u ishrani kao novi vezujući partner c-fikocijanina: fluorimetrijska studija en ABSTRACT C-Phycocyanin (C-PC) is a phycobiliprotein from cyanobacteria, where it harvests light energy that is then transferred to chlorophylls during photosynthesis. It has an intense blue color due to a covalently bonded tetrapyrrole chromophore, and owing to this property is used in the food industry as a good natural alternative for food coloring. In addition to its coloring properties, C-PC has anti-inflammatory, antioxidant, anti-cancer, and immune-enhancing effects that qualify it as a dietary supplement already included in various formulations, mainly Spirulina extract powders. Since it is used as a food colorant and as a dietary supplement, it may interact with food ingredients, affecting its stability, digestibility, or antioxidant properties. Palmitic acid and linoleic acid (which can be metabolized to linolenic acid) are abundant in meat, milk, and edible oils, so that they could interact with C-PC. C- Phycocyanin isolated from the cyanobacterium Arthrospira platensis (Spirulina) was incubated with increasing concentrations of these three fatty acids, and its fluorescence intensity was monitored. Incubation resulted in a fluorescence quenching effect, indicating that binding had occurred. The binding equations indicated that the association constants were of the same order of magnitude and that the number of approximate binding sites was more than one (Ka = 4.64 x 10⁴ M-¹, n = 1.5 for linoleic acid; Ka = 2.88 x 10⁴ M–¹, n = 1.9 for linolenic acid; Ka = 0.44 x 10⁴ M–¹, n = 0.8 for palmitic acid). This moderate interaction between C-PC and fatty acids could influence its behavior as a nutraceutical and food colorant. fatty acids; C-phycocyanin; fluorimetry; binding constant 1552102 https://euroanalysis2023.ch/Docs/EA23_Abstract_Book.pdf 2023-09-14T13:05:28.423Z 44 yes 46 Miloš Šunderić University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia doktor biohemijskih nauka 0000-0002-0940-9481 Luka Veličković Center of Excellence for Molecular Food Sciences & Department of Biochemistry, University of Belgrade-Faculty of Chemistry, Belgrade, Serbia person Nikola Gligorijević Institute of Chemistry, Technology, and Metallurgy, National Institute of the Republic of Serbia, Department of Chemistry, University of Belgrade, Serbia person Ljubodrag Aleksić Center of Excellence for Molecular Food Sciences & Department of Biochemistry, University of Belgrade-Faculty of Chemistry, Belgrade, Serbia person Milan Nikolić Center of Excellence for Molecular Food Sciences & Department of Biochemistry, University of Belgrade-Faculty of Chemistry, Belgrade, Serbia person Marija Takić Centre of Research Excellence in Nutrition and Metabolism, Institute for Medical Research, National Institute of Republic of Serbia, University of Belgrade, Belgrade, Serbia person Simeon Minić Center of Excellence for Molecular Food Sciences & Department of Biochemistry, University of Belgrade-Faculty of Chemistry, Belgrade, Serbia person application/pdf 4200461 https://phaidrabg.bg.ac.rs/o:30810 no yes 18 70 92000004 11A29 11A2905 11A19 11A24 11A2404 11A25 EUROANALYSIS 2023-Analytical Probing of Complex Systems Swiss Chemical Society-Division of Analytical Chemistry 2023