o:30809 Ex vivo and in vivo antioxidant activity of β-hydroxy-β-arylalkanoic acids en ABSTRACT The interplay between oxidative stress and inflammation is implicated in many chronic diseases including Alzheimer`s disease, cardiovascular diseases, diabetes and cancer. Thirteen β-hydroxy-β-arylalkanoic acids were previously synthesized and evaluated for their anti-inflammatory activity. The aim of this study was to asses ex vivo antioxidant activity of synthesized acids, as well as ibuprofen and to identify the compounds with the most promising results for further investigation on their capacity to counteract in vivo oxidative stress triggered by inflammation. The antioxidant potential of tested compounds was evaluated by determining the concentrations of total antioxidative status, total oxidative status, prooxidant antioxidant balance and the total sulfhydryl groups. Z score statistics were used to calculate the summary scores for antioxidative activity, prooxidative activity and oxy score. The tested compounds and ibuprofen demonstrated mild prooxidative activity ex vivo. Seven acids with substituents on one benzene ring exhibited better results than ibuprofen and were selected for in vivo testing. In vivo results demonstrated better antioxidant protection compared to ex vivo results. Compound g which contains nitro group on the benzene ring demonstrated the lowest oxy score, and four compounds exhibited better results than ibuprofen. Inflammation, oxidative stress, oxy score, anti-inflammatory compounds 1552102 https://www.pjps.pk/uploads/2023/09/1693548895.pdf 2023-09-14T12:17:26.741Z 44 yes 46 Branko Subošić Biochemical laboratory, University Children’s Hospital, Belgrade, Serbia Jelena Kotur-Stevuljević Department of Medical Biochemistry, University of Belgrade-Faculty of Pharmacy, Belgrade, Serbia person Jasmina Brborić Department of Pharmaceutical Chemistry, University of Belgrade-Faculty of Pharmacy, Belgrade, Serbia person Tamara Janković University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia person 0000-0002-8188-0921 Marina Milenković Department of Microbiology and Immunology, University of Belgrade-Faculty of Pharmacy, Serbia person Branka Ivković Department of Pharmaceutical Chemistry, University of Belgrade-Faculty of Pharmacy, Belgrade, Serbia person Jelena Kostadinović Department of Medical Oncology, CHC Bežanijska kosa, Belgrade, Serbia person Jelena Savić Department of Pharmaceutical Chemistry, University of Belgrade-Faculty of Pharmacy, Belgrade, Serbia person application/pdf 580385 https://phaidrabg.bg.ac.rs/o:30809 no yes 1 70 92000001 11A13 11A1313 11A13 11A1305 11A29 11A2903 11A13 11A1314 Pakistan Journal of Pharmaceutical Sciences 36 5 1367 1374 Faculty of Pharmacy, University of Karachi 2023