2023 Ex vivo and in vivo antioxidant activity of β-hydroxy-β-arylalkanoic acids https://phaidrabg.bg.ac.rs/o:30809 ABSTRACT The interplay between oxidative stress and inflammation is implicated in many chronic diseases including Alzheimer`s disease, cardiovascular diseases, diabetes and cancer. Thirteen β-hydroxy-β-arylalkanoic acids were previously synthesized and evaluated for their anti-inflammatory activity. The aim of this study was to asses ex vivo antioxidant activity of synthesized acids, as well as ibuprofen and to identify the compounds with the most promising results for further investigation on their capacity to counteract in vivo oxidative stress triggered by inflammation. The antioxidant potential of tested compounds was evaluated by determining the concentrations of total antioxidative status, total oxidative status, prooxidant antioxidant balance and the total sulfhydryl groups. Z score statistics were used to calculate the summary scores for antioxidative activity, prooxidative activity and oxy score. The tested compounds and ibuprofen demonstrated mild prooxidative activity ex vivo. Seven acids with substituents on one benzene ring exhibited better results than ibuprofen and were selected for in vivo testing. In vivo results demonstrated better antioxidant protection compared to ex vivo results. Compound g which contains nitro group on the benzene ring demonstrated the lowest oxy score, and four compounds exhibited better results than ibuprofen. Inflammation, oxidative stress, oxy score, anti-inflammatory compounds Pakistan Journal of Pharmaceutical Sciences 36(5) info:eu-repo/semantics/article Subošić, Branko Kotur-Stevuljević, Jelena Brborić, Jasmina Janković, Tamara Milenković, Marina Ivković, Branka Kostadinović, Jelena Savić, Jelena application/pdf 580385 bytes eng All rights reserved Faculty of Pharmacy, University of Karachi