o:30789 Lectin-based glycoprotein microarray-a tool for glycan analysis of gestational diabetes mellitus samples Glikoproteinski mikroerej baziran na lektinima-alat za glikansku analizu uzoraka u gestacijskom dijabetesu en ABSTRACT According to WHO approximately 422 million of people worldwide suffer from diabetes. This number increases every year. Gestational diabetes mellitus (GDM) is metabolic disease occurring during pregnancy, which can lead to several outcomes for mother and the child. Glycans provide many important biological functions and appear as a potential tool to distinguish diabetes type 1 from type 2. Based on that, we focused on the glycans as possible biomarkers of GDM. We compared glycan composition of serum, plasma and fibrinogen samples of healthy pregnant women and women with GDM. Samples were analysed using our lectin-based microarray method. Significant differences were found in serum and plasma samples. GDM serum samples showed significant drop of N-acetylgalactosamine (RCA), N-glycans with core fucose (LCA) and increase of glycan structures with siatie acid (MALII). GDM plasma samples indicated a significantly higher fucosylation (AAL). Found differences (GDM/non-GDM) in glycan composition can play important role for identification of potential glycan biomarkers, which can be useful in research and early diagnostics of GDM. glycan, Gestational diabetes mellitus, glycan biomarkers 1552100 978-80-280-0040-0 2023-09-13T11:45:35.888Z 44 yes 46 Lucia Pažitná lnstitute of Chemistry SAS, Bratislava, Slovakia Kristína Kianičková lnstitute of Chemistry SAS, Bratislava, Slovakia person Paras Kundalia lnstitute of Chemistry SAS, Bratislava, Slovakia person Zorana Dobrijević University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia doktor nauka-biološke nauke person 0000-0002-4652-7719 Dragana Robajac University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia doktor bioloških nauka person 0000-0002-6423-4645 Nikola Gligorijević University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia person Goran Miljuš University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia doktor biohemijskih nauka person 0000-0001-6471-8711 Ana Penezić University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia doktor biohemijskih nauka person 0000-0002-2539-4507 Miloš Šunderić University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia doktor biohemijskih nauka person 0000-0002-0940-9481 Vesna Mandić Marković University of Belgrade, Faculty of Medicine, Serbia person Ognjen Radojičić Gynecology and Obstetrics Clinic "Narodni front", Belgrade, Serbia person Željko Miković Gynecology and Obstetrics Clinic "Narodni front", Belgrade, Serbia person Olgica Nedić University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia doktor nauka-biološke nauke person 0000-0003-2042-0056 Jaroslav Katrlík lnstitute of Chemistry SAS, Bratislava, Slovakia person application/pdf 392052 https://phaidrabg.bg.ac.rs/o:30789 no yes 18 70 92000004 11A29 11A2905 11A32 The Biomania Student Scientific Meeting 2022-Book of Abstracts Masaryk University 2022