o:30747 Association of acute Babesia canis infection and serum lipid, lipoprotein and apoprotein concentrations in dogs Povezanost akutne infekcije Babesia-om canis sa nivoima serumskih lipida, lipoproteina i apoproteina kod pasa en ABSTRACT Background: Babesia canis infection induces a marked acute phase response (APR) that might be associated with alteration in lipid and lipoprotein metabolism and disease prognosis. Hypothesis: Dogs with B. canis-induced APR develop dyslipidemia with altered lipoprotein concentration and morphology. Animals: Twenty-nine client-owned dogs with acute B. canis infection and 10 clinically healthy control dogs. Methods: Observational cross-sectional study. Serum amyloid A (SAA) was measured using ELISA. Cholesterol, phospholipids, and triglycerides were determined biochemically. Lipoproteins were separated using agarose gel electrophoresis. Lipoprotein diameter was assessed by polyacrylamide gradient gel electrophoresis; correlation with ApoA-1 (radioimmunoassay) and SAA was determined. Results: Dogs with B. canis infection had a marked APR (median SAA, 168.3 μg/mL; range, 98.1-716.2 μg/mL) compared with controls (3.2 μg/mL, 2.0-4.2 μg/mL) (P< .001). Dogs with B. canis infection had significantly lower median cholesterol (4.79 mmol/L, 1.89-7.64 mmol/L versus 6.15 mmol/L, 4.2-7.4 mmol/L) (P = .02), phospholipid (4.64 mmol/L, 2.6-6.6 mmol/L versus 5.72 mmol/L, 4.68-7.0 mmol/L) (P = .02), and α-lipoproteins (77.5%, 27.7%-93.5% versus 89.2%, 75.1%-93.5%) (P = .04), and higher ApoA-1 (1.36 U, 0.8-2.56 U versus 0.95 U, 0.73-1.54 U) concentrations (P = .02). Serum amyloid A correlated with high-density lipoproteins (HDLs) diameter (rho = .43; P = .03) and ApoA-1 (rho = .63, P < .001). Conclusions and clinical importance: Major changes associated with B. canis-induced APR in dogs are related to concentration, composition, and morphology of HDL particles pointing to an altered reverse cholesterol transport. Parallel ApoA-1 and SAA concentration increase is a unique still unexplained pathophysiological finding. acute phase response; apolipoprotein A-1; high-density lipoprotein; lipoprotein diameter; serum amyloid A 1552099 10.1111/jvim.15537 2023-09-07T11:20:02.775Z 44 yes 46 Zorana Milanović Faculty of Veterinary Medicine, University of Belgrade, Serbia Jelena Vekić Faculty of Pharmacy, University of Belgrade, Serbia person Vladimir Radonjić Small animal practice "Petrovac", Belgrade, Serbia person Anja Ilić Božović Faculty of Veterinary Medicine, University of Belgrade, Serbia person Aleksandra Zeljković Faculty of Pharmacy, University of Belgrade, Serbia person Jelena Janac Faculty of Pharmacy, University of Belgrade, Serbia person Vesna Spasojević-Kalimanovska Faculty of Pharmacy, University of Belgrade, Serbia person Jesse Buch IDEXX Laboratories, Westbrook, Maine person Ramaswamy Chandrashekar IDEXX Laboratories, Westbrook, Maine person Žanka Bojić-Trbojević University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia doktor bioloških nauka person 0000-0001-5619-1027 Ljiljana Hajduković University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbi doktor biohemijskih nauka person 0000-0003-0720-2345 Mary M. Christopher Department of Pathology, Microbiology, and Immunology, School of Veterinary Medicine, University of California, Davis, California person Milica Kovačević Filipović Faculty of Veterinary Medicine, University of Belgrade, Serbia person application/pdf 1027195 https://phaidrabg.bg.ac.rs/o:30747 no yes 18 70 92000001 11A10 11A13 11A29 11A2903 Journal of Veterinary Internal Medicine 33 4 1686 1694 ACVIM American College of Veterinary Internal Medicine 2019