https://phaidrabg.bg.ac.rs/o:30747 doi:10.1111/jvim.15537 acute phase response; apolipoprotein A-1; high-density lipoprotein; lipoprotein diameter; serum amyloid A application/pdf 1027195 bytes http://creativecommons.org/licenses/by-nc-nd/4.0/legalcode info:eu-repo/semantics/article ACVIM American College of Veterinary Internal Medicine ABSTRACT Background: Babesia canis infection induces a marked acute phase response (APR) that might be associated with alteration in lipid and lipoprotein metabolism and disease prognosis. Hypothesis: Dogs with B. canis-induced APR develop dyslipidemia with altered lipoprotein concentration and morphology. Animals: Twenty-nine client-owned dogs with acute B. canis infection and 10 clinically healthy control dogs. Methods: Observational cross-sectional study. Serum amyloid A (SAA) was measured using ELISA. Cholesterol, phospholipids, and triglycerides were determined biochemically. Lipoproteins were separated using agarose gel electrophoresis. Lipoprotein diameter was assessed by polyacrylamide gradient gel electrophoresis; correlation with ApoA-1 (radioimmunoassay) and SAA was determined. Results: Dogs with B. canis infection had a marked APR (median SAA, 168.3 μg/mL; range, 98.1-716.2 μg/mL) compared with controls (3.2 μg/mL, 2.0-4.2 μg/mL) (P< .001). Dogs with B. canis infection had significantly lower median cholesterol (4.79 mmol/L, 1.89-7.64 mmol/L versus 6.15 mmol/L, 4.2-7.4 mmol/L) (P = .02), phospholipid (4.64 mmol/L, 2.6-6.6 mmol/L versus 5.72 mmol/L, 4.68-7.0 mmol/L) (P = .02), and α-lipoproteins (77.5%, 27.7%-93.5% versus 89.2%, 75.1%-93.5%) (P = .04), and higher ApoA-1 (1.36 U, 0.8-2.56 U versus 0.95 U, 0.73-1.54 U) concentrations (P = .02). Serum amyloid A correlated with high-density lipoproteins (HDLs) diameter (rho = .43; P = .03) and ApoA-1 (rho = .63, P < .001). Conclusions and clinical importance: Major changes associated with B. canis-induced APR in dogs are related to concentration, composition, and morphology of HDL particles pointing to an altered reverse cholesterol transport. Parallel ApoA-1 and SAA concentration increase is a unique still unexplained pathophysiological finding. Journal of Veterinary Internal Medicine 33(4) Milanović, Zorana Vekić, Jelena Radonjić, Vladimir Ilić Božović, Anja Zeljković, Aleksandra Janac, Jelena Spasojević-Kalimanovska, Vesna Buch, Jesse Chandrashekar, Ramaswamy Bojić-Trbojević, Žanka Hajduković, Ljiljana Christopher, Mary M. Kovačević Filipović, Milica 2019 eng Association of acute Babesia canis infection and serum lipid, lipoprotein and apoprotein concentrations in dogs