o:29819 Electrospun composite nanofibers containing biocompatible inorganic tungsten disulfide nanoparticles Kompozitna nanovlakna dobijena elektrospiningom koja sadrže biokompatibilne nanočestice volfram disulfida en ABSTRACT Tungsten disulfide nanoparticles (WS2) emerged as excellent theranostics tool due to their exquisite optical properties and wide surface available for bioconjugation. However, their controlled delivery in vivo remains a challenge. Poly (lactic-co-glycolic acid) (PLGA) nanofibers are widely used as preferred carriers for the controlled release of drugs due to their biodegradability and their easy formation and preparation. In this study, the inorganic WS2 nanoparticles demonstrated lack of cytotoxicity towards human peripheral blood mononuclear cells (PBMC) in vitro up to 100 µg/ml. Although these nanoparticles were easily internalized by monocytes, they did not modulate PHA induced proliferation of PBMCs significantly. After loading of WS2 with fluorescein isothiocyanate (FITC) as a model drug, they displayed similar cytotoxic profile in culture with PBMCs. PLGA composite nanofibers containing FITC-loaded WS2 were prepared through an electrospinning solution technique. Field-emission scanning electron microscopy and in-situ scanning probe microscopy imaging were used to examine the morphology of the biocompatible nanoparticles and electrospun nanofibers, respectively. The distribution of fluorescent nanoparticles in the composite nanofibers was evaluated by fluorescence microscopy. Cumulatively, the electrospun composite nanofibers incorporated with biocompatible WS2 inorganic nanoparticles represent new attractive theranostics platform, enabling a well-controlled delivery of bioactive molecules. 1552100 978-86-919111-6-4 91552100 44447497 2023-05-25T18:30:34.407Z 44 yes 46 Dušica B. Stojanović University of Belgrade, Faculty of Technology and Metallurgy, Department of Materials Science and Engineering, Serbia Sergej Tomić University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia person 0000-0003-2570-1295 Marina Bekić University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia person 0000-0002-6945-4241 Snežana Zečević University of East Sarajevo, Medical Faculty of Foča, Foča, Bosnia and Herzegovina person Darinka Popović University of East Sarajevo, Medical Faculty of Foča, Foča, Bosnia and Herzegovina person Miodrag Čolić University of East Sarajevo, Medical Faculty of Foča, Foča, Bosnia and Herzegovina person Petar S. Uskoković University of Belgrade, Faculty of Technology and Metallurgy, Department of Materials Science and Engineering, Serbia person application/pdf 9563 https://phaidrabg.bg.ac.rs/o:29819 no yes 18 70 92000004 11A41 11A29 11A2904 The twenty-second annual conference YUCOMAT 2021-Book of Abstracts 128 Materials research society of Serbia-MRSS 2021