o:29427 Disulfiram with metformin inhibit hamster fibrosarcoma growth en Abstract: We investigated the anticancer effect of disulfiram and metformin combination on fibrosarcoma in hamsters. Syrian golden hamsters of both sexes (~70 g) were randomly allocated to control and experimental groups (8 animals per group). In all groups, 2 × 10 6 BHK-21/C13 cells in 1 ml were injected subcutaneously into the animals’ backs. Peroral treatments were carried out with disulfiram 50 mg/kg daily, or with metformin 500 mg/kg daily, or with their combination. Validation grups were treated by double doses of the single therapy, via a gastric probe after tumor inoculation. In the course of experiment, changes in tumor growth of each animal were recorded daily. To obtain volume estimates, we used calipers to measure the tumor diameters.The animals were sacrificed 19 days post inoculation. Blood samples were collected for hematological and biochemical analyses, and the main organs were toxicologically tested. The tumors were excised and weighed, and their diameters and volumes were measured. The tumor samples were pathohistologically and immunohistochemically assessed (Ki-67, PCNA, CD34, CD31, COX4, Cytochrome C, GLUT1, iNOS). The combination of disulfiram and metformin significantly inhibited fibrosarcoma growth in hamsters without toxicity, compared to monotherapy or control. The single treatments did not show significant antisarcoma effect. Administration of disulfiram with metformin might be an effective and safe approach in novel nontoxic adjuvant anticancer treatment and relapse prevention antitumor therapy 1552100 978-86-7220-124-6 1552099 http://www.bds.org.rs/download/SBS_Conference_11_2022.pdf 2023-05-15T09:09:17.731Z 44 no 46 Kosta J. Popović 0000-0001-7895-9009 Dušica J. Popović Državni univerzitet u Novom Pazaru person 0000-0001-7880-0874 Dejan Miljković person 0000-0002-7739-5202 Dušan Lalošević person 0000-0002-0760-9180 Ivan Čapo person 0000-0001-7531-5099 Mihalj Poša person 0000-0002-8044-2655 Zana Dolićanin Državni univerzitet u Novom Pazaru person 0000-0003-0457-3087 Jovan Popović person 0000-0002-6790-8668 application/pdf 151509 https://phaidrabg.bg.ac.rs/o:29427 no yes 1 70 92000004 20A01 Biochemical Society Eleventh Conference, Scientific meeting of an international Serbian character “Amazing Biochemistry” 22-23.09.2022. Novi Sad, Serbia. Proceedings. p. 123 (M34) 11 123 123 2022