o:27852 MiR-375 and miR-21 as potential biomarkers of prostate cancer: comparison of matching samples of plasma and exosomes MiR-375 i miR-21 kao potencijalni biomarkeri karcinoma prostate: poredjenje sparenih uzoraka plazme i egzozoma en ABSTRACT MiR-21 and miR-375 have been reported as dysregulated in prostate cancer (PCa) in multiple previous studies. Still, variable or even opposing data for the expression of these microRNAs in PCa were found, and their potential biomarker properties remain elusive. In an attempt to clarify their significance as PCa biomarkers, as well as to compare different types of specimens as a source of relevant microRNAs, we used plasma and matching plasma-derived exosomes from patients with PCa and patients with benign prostatic hyperplasia (BPH). Plasma and exosomes were obtained from 34 patients with PCa and 34 patients with BPH, and their levels of expression of miR-21 and miR-375 were determined by RT-qPCR. We found no significant difference in the level of expression of these microRNAs in plasma and exosomes between patients with PCa and BPH. The level of exosomal miR-21 was elevated in PCa patients with high serum PSA values, as well as in patients with aggressive PCa, while for plasma samples, the results remained insignificant. For miR-375, we did not find an association with the values of standard prognostic parameters of PCa, nor with cancer aggressiveness. Therefore, our results support the potential prognostic role of exosomal miR-21 expression levels in PCa. prostate cancer, exosomes, plasma, microRNAs, miR-21, miR-375 1552099 10.3390/genes13122320 2022-12-27T12:36:26.154Z 44 yes 46 Suzana Matijašević Joković University of Belgrade, Faculty of Biology, Centre for Human Molecular Genetics, Serbia Goran Brajušković University of Belgrade, Faculty of Biology, Centre for Human Molecular Genetics, Serbia person Dušanka Savić-Pavićević University of Belgrade, Faculty of Biology, Centre for Human Molecular Genetics, Serbia person Ivan Vuković Clinical Center of Serbia, Clinic of Urology, Belgrade, Serbia person Aleksandra Korać University of Belgrade, Faculty of Biology, Centre for Human Molecular Genetics, Serbia person Milica Popović University of Belgrade, Faculty of Chemistry, Serbia person Lidija Filipović University of Belgrade, Faculty of Chemistry, Innovative Centre, Serbia person Nevena Kotarac University of Belgrade, Faculty of Biology, Centre for Human Molecular Genetics, Serbia person Zorana Dobrijević University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia doktor nauka-biološke nauke person 0000-0002-4652-7719 application/pdf 1496938 https://phaidrabg.bg.ac.rs/o:27852 no yes 16 70 92000001 11A02 11A29 11A2905 11A19 11A1907 11A19 Genes 13 12 MDPI 2022