
<ns0:uwmetadata xmlns:ns0="http://phaidra.univie.ac.at/XML/metadata/V1.0" xmlns:ns1="http://phaidra.univie.ac.at/XML/metadata/lom/V1.0" xmlns:ns10="http://phaidra.univie.ac.at/XML/metadata/provenience/V1.0" xmlns:ns11="http://phaidra.univie.ac.at/XML/metadata/provenience/V1.0/entity" xmlns:ns12="http://phaidra.univie.ac.at/XML/metadata/digitalbook/V1.0" xmlns:ns13="http://phaidra.univie.ac.at/XML/metadata/etheses/V1.0" xmlns:ns2="http://phaidra.univie.ac.at/XML/metadata/extended/V1.0" xmlns:ns3="http://phaidra.univie.ac.at/XML/metadata/lom/V1.0/entity" xmlns:ns4="http://phaidra.univie.ac.at/XML/metadata/lom/V1.0/requirement" xmlns:ns5="http://phaidra.univie.ac.at/XML/metadata/lom/V1.0/educational" xmlns:ns6="http://phaidra.univie.ac.at/XML/metadata/lom/V1.0/annotation" xmlns:ns7="http://phaidra.univie.ac.at/XML/metadata/lom/V1.0/classification" xmlns:ns8="http://phaidra.univie.ac.at/XML/metadata/lom/V1.0/organization" xmlns:ns9="http://phaidra.univie.ac.at/XML/metadata/histkult/V1.0">
  <ns1:general>
    <ns1:identifier>o:27327</ns1:identifier>
    <ns1:title language="en">Efficacy of anaerosol-resistant pepsin powder used in artificial digestion for the detection of Trichinella larvae in meat</ns1:title>
    <ns1:language>en</ns1:language>
    <ns1:description language="en">ABSTRACT
Trichinellosis is an important worldwide foodborne zoonosis. The gold standard test to detect Trichinella spp. larvae in muscle samples of animals intended for human consumption is the artificial digestion method. Handling and dispensing of conventional pepsin powder present significant safety risks for analysts. The use of pepsin powder that is resistant to aerosolization should alleviate these safety concerns. The aim of this study was to compare the efficacy of an aerosol-resistant pepsin powder to conventional pepsin powder in the artificial digestion method. Proficiency samples of pork diaphragm containing specific numbers of viable Trichinella spiralis larvae were tested in two laboratories. The results revealed that aerosol-resistant pepsin was simple, effective and convenient to use, and showed good solubility and larval recovery that met the requirements of the European Union regulation EU 2015/1375. Overall, the efficacy of the aerosol-resistant pepsin was comparable to the conventional pepsin and safer for analysts.</ns1:description>
    <ns1:keyword language="en">Trichinella, digestion method, pepsin</ns1:keyword>
    <ns2:identifiers>
      <ns2:resource>1552099</ns2:resource>
      <ns2:identifier>10.1017/S0022149X2200061X</ns2:identifier>
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  <ns1:lifecycle>
    <ns1:upload_date>2022-12-26T07:58:38.404Z</ns1:upload_date>
    <ns1:status>44</ns1:status>
    <ns2:peer_reviewed>yes</ns2:peer_reviewed>
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      <ns1:role>46</ns1:role>
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        <ns3:firstname>Saša</ns3:firstname>
        <ns3:lastname>Vasilev</ns3:lastname>
        <ns3:institution>University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia</ns3:institution>
        <ns3:title1>doktor medicinskih nauka-veterina</ns3:title1>
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        <ns3:firstname>Dragan</ns3:firstname>
        <ns3:lastname>Vasilev</ns3:lastname>
        <ns3:institution>University of Belgrade, Faculty of Veterinary Medicine, Serbia</ns3:institution>
        <ns3:type>person</ns3:type>
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      <ns1:entity seq="2">
        <ns3:firstname>Ljiljana</ns3:firstname>
        <ns3:lastname>Sofronić Milosavljević</ns3:lastname>
        <ns3:institution>University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia</ns3:institution>
        <ns3:title1>doktor medicinskih nauka</ns3:title1>
        <ns3:type>person</ns3:type>
        <ns3:orcid>0000-0002-2721-125X</ns3:orcid>
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        <ns3:firstname>Nedjeljko</ns3:firstname>
        <ns3:lastname>Karabasil</ns3:lastname>
        <ns3:institution>University of Belgrade, Faculty of Veterinary Medicine, Serbia</ns3:institution>
        <ns3:type>person</ns3:type>
      </ns1:entity>
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        <ns3:firstname>Ljiljana</ns3:firstname>
        <ns3:lastname>Sabljić</ns3:lastname>
        <ns3:institution>University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia</ns3:institution>
        <ns3:title1>doktor veterinarske medicine</ns3:title1>
        <ns3:type>person</ns3:type>
        <ns3:orcid>0000-0001-6027-591X</ns3:orcid>
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        <ns3:firstname>Ivana</ns3:firstname>
        <ns3:lastname>Mitić</ns3:lastname>
        <ns3:institution>University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia</ns3:institution>
        <ns3:title1>doktor medicinskih nauka</ns3:title1>
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        <ns3:firstname>Branko</ns3:firstname>
        <ns3:lastname>Suvajdžić</ns3:lastname>
        <ns3:institution>University of Belgrade, Faculty of Veterinary Medicine, Serbia</ns3:institution>
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  <ns1:technical>
    <ns1:format>application/pdf</ns1:format>
    <ns1:size>107571</ns1:size>
    <ns1:location>https://phaidrabg.bg.ac.rs/o:27327</ns1:location>
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    <ns1:cost>no</ns1:cost>
    <ns1:copyright>yes</ns1:copyright>
    <ns1:license>16</ns1:license>
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    <ns1:purpose>70</ns1:purpose>
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      <ns8:faculty>11A29</ns8:faculty>
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      <ns8:faculty>11A10</ns8:faculty>
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  <ns12:digitalbook>
    <ns12:name_magazine language="en">Journal of Helminthology</ns12:name_magazine>
    <ns12:volume>96</ns12:volume>
    <ns12:publisher>Cambridge University Press</ns12:publisher>
    <ns12:releaseyear>2022</ns12:releaseyear>
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