o:26026 Strawberry (Fragaria ananassa duch.) Alba extract attenuates DNA damage in lymphocytes of patients with Alzheimer’s disease en ABSTRACT: Increased levels of oxidative stress and oxidative DNA damage are common features in the pathology of Alzheimer’s disease (AD) found in neurons and peripheral cells like peripheral blood lymphocytes (PBL). Natural products such as strawberry cultivar Alba are an important source of bioactive nutrients that could help in lowering both the oxidative stress and DNA damage levels. The objective was to estimate the effects of Alba extract on DNA damage in peripheral blood lymphocytes of sporadic AD (aged 60–84 years) patients, and healthy elderly (aged 69–83 years) and young (aged 21–30 years) individuals in in vitro conditions. Comet assay was used as a sensitive technique for the evaluation of PBL DNA damage levels. Reduction of basal DNA damage level in PBL was shown in the young group after the incubation with Alba extract ranging from 25 to 200 μg/ml, with 100 μg/ml being the most effective concentration. Selected Alba extract of 100 μg/ml was further used for PBL treatment of AD and healthy elderly age matched group, displaying potential to significantly attenuate DNA damage levels in both groups (p <.05). Alba extract displayed biological activity against oxidative DNA damage, suggesting that its functional ingredients may have beneficial health effects. Practical applications: The data obtained in this preliminary study displayed that strawberry Alba extract is efficient against DNA damage induced by endogenous and exogenous oxidative stress in peripheral blood lymphocytes of Alzheimer`s disease in vitro. An active area of future research of Alba cultivar should be to determine the trials in in vivo systems. Our findings also suggest that Alba cultivar’s functional ingredients potentially may have beneficial health effects in AD. Sažetak ageing, comet assay, DNA damage, neurodegenerative disease 1552099 10.1111/jfbc.13637 2022-06-13T08:55:01.498Z 44 yes 46 Lada Živković University of Belgrade, Faculty of Pharmacy, Department of Pathobiology, Serbia Biljana Spremo-Potparević University of Belgrade, Faculty of Pharmacy, Department of Pathobiology, Serbia person George Perry The University of Texas, College of Sciences, USA person Mirjana Zlatković-Švenda University of Belgrade, School of Medicine, Clinical Rheumatology, Institute of Rheumatology, Serbia person Tamara Yuliett Forbes-Hernández University of Vigo, Nutrition and Food Science Group, Department of Analytical and Food Chemistry, CITACA, CACTI, Spain person Dragana Dekanski Biomedical Research, R&D Institute, Belgrade, Serbia doktor medicinskih nauka person 0000-0001-8467-4165 Andrea Čabarkapa-Pirković University of Belgrade, Faculty of Pharmacy, Department of Pathobiology, Serbia doktor bioloških nauka person 0000-0002-1182-4895 Vladan Bajić University of Belgrade, Institute for Nuclear Research VINČA, The Laboratory for Radiobiology and Molecular Genetics, Serbia person application/pdf 73756 https://phaidrabg.bg.ac.rs/o:26026 no yes 1 70 92000001 11A13 11A28 11A2810 11A32 Journal of Food Biochemistry 45 4 Wiley 2021