o:25999 Caffeic acid protects human trophoblast HTR-8/SVneo cells from H2O2-induced oxidative stress and genotoxicity en ABSTRACT Caffeic acid is highlighted as one of the major phenolic compounds present in foods with known antioxidant activity. This phenolic is among commonly consumed substances in everyday diet of pregnant women. However, there is not enough information on its effects during pregnancy, especially the most vulnerable early stage. Extravillous trophoblast cells are specific cells of the placenta that come in direct contact with maternal uterine tissue. Through this study we investigated the cytoprotective effects of caffeic acid on H2O2-induced oxidative damage in first trimester extravillous trophoblast cell line HTR-8/SVneo. Investigated concentrations (1-100 μM) of caffeic acid showed neither cytotoxic nor genotoxic effects on HTR-8/SVneo cells. The treatment with caffeic acid 100 μM significantly increased the percentage of cells in G2/M phase of the cell cycle, compared to non-treated cells. Pretreatment with caffeic acid (10 and 100 μM) attenuated oxidative DNA damage significantly, reduced cytotoxicity, protein and lipid peroxidation, and restored antioxidant capacity in trophoblast cells following H2O2 exposure. This beneficial outcome is probably mediated by the augmentation of GSH and effective ROS scavenging by caffeic acid. These promising results require further investigations to reveal the additional mechanisms/pathways and confirmation through studies in vivo. caffeic acid, cytotoxicity, HTR-8/SVneo cells, oxidative DNA damage 1552099 10.1016/j.fct.2022.112993 2022-06-09T19:11:43.737Z 44 yes 46 Sanja Kostić University of Belgrade, Faculty of Medicine, Clinic of Obstetrics and Gynecology, Clinical Center of Serbia, Serbia Milica Jovanović Krivokuća University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia doktor bioloških nauka person 0000-0001-8292-4853 Svetlana Vrzić-Petronijević University of Belgrade, Faculty of Medicine, Clinic of Obstetrics and Gynecology, Clinical Center of Serbia, Serbia person Mirjana Nacka-Aleksić University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia doktor medicinskih nauka-farmacija person 0000-0003-0750-9347 Sunčica Borozan University of Belgrade, Faculty of Veterinary medicine, Department of Chemistry, Serbia person Dragana Dekanski University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia doktor medicinskih nauka person 0000-0001-8467-4165 Andrea Pirković University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia doktor bioloških nauka person 0000-0002-1182-4895 Aleksandra Vilotić University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia doktor bioloških nauka person 0000-0002-0898-5401 application/pdf 61635 https://phaidrabg.bg.ac.rs/o:25999 no yes 1 70 92000001 11A32 11A3260 11A29 11A2901 11A10 Food and Chemical Toxicology 163 Elsevier 2022