o:25899 Lectin-Based Protein Microarray for the Glycan Analysis of Colorectal Cancer Biomarkers: The Insulin-Like Growth Factor System Lektinski mikroerej za analizu biomarkera kolorektalnog kancera: Sistem faktora rasta sličnih insulinu en ABSTRACT Lectin-based protein microarrays are used for glycoprofiling of various kinds of biological samples. Here we describe lectin-based microarray assay in the reverse-phase format where glycoprotein samples are spotted onto microarray slide and then are incubated with set of lectins. This configuration allows high-throughput screening of a large cohort of samples by a set of lectins without need of separation of glycans from glycoproteins. We applied the described method for glycan analysis of glycoprotein biomarkers of colorectal cancer associated with the insulin-like growth factor system. Sažetak biomarker, cancer, glycosylation, insulin-like growth factor system, lectin-based protein microarray 1552099 10.1007/978-1-0716-2148-6_13 2022-06-03T12:05:00.504Z 44 yes 46 Dragana Robajac University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia doktor biohemijskih nauka 0000-0002-6423-4645 Jaroslav Katrlík Slovak Academy of Sciences, Bratislava, Department of Glycobiotechnology, Institute of Chemistry, Slovakia person Olgica Nedić University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia doktor biohemijskih nauka person 0000-0003-2042-0056 Peter Gemeiner Slovak Academy of Sciences, Bratislava, Department of Glycobiotechnology, Institute of Chemistry, Slovakia person Goran Miljuš University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia doktor biohemijskih nauka person 0000-0001-6417-8711 Miloš Šunderić University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia doktor biohemijskih nauka person 0000-0002-0940-9481 Martina Križáková Slovak Academy of Sciences, Bratislava, Department of Glycobiotechnology, Institute of Chemistry, Slovakia person application/pdf 5055 https://phaidrabg.bg.ac.rs/o:25899 no yes 1 70 92000001 11A29 11A2905 Methods in Molecular Biology 2460 207 222 Springer Science+Business Media, LLC 2022