Robajac, Dragana Katrlík, Jaroslav Nedić, Olgica Gemeiner, Peter Miljuš, Goran Šunderić, Miloš Križáková, Martina eng ABSTRACT Lectin-based protein microarrays are used for glycoprofiling of various kinds of biological samples. Here we describe lectin-based microarray assay in the reverse-phase format where glycoprotein samples are spotted onto microarray slide and then are incubated with set of lectins. This configuration allows high-throughput screening of a large cohort of samples by a set of lectins without need of separation of glycans from glycoproteins. We applied the described method for glycan analysis of glycoprotein biomarkers of colorectal cancer associated with the insulin-like growth factor system. Sažetak https://phaidrabg.bg.ac.rs/o:25899 doi:10.1007/978-1-0716-2148-6_13 info:eu-repo/semantics/article Springer Science+Business Media, LLC application/pdf 5055 bytes Lectin-Based Protein Microarray for the Glycan Analysis of Colorectal Cancer Biomarkers: The Insulin-Like Growth Factor System All rights reserved biomarker, cancer, glycosylation, insulin-like growth factor system, lectin-based protein microarray Methods in Molecular Biology 2460 2022