o:25684 Trichinella spiralis excretory-secretory products downregulate MMP-9 in Dark Agouti rats affected by experimental autoimmune encephalomyelitis Ekskretorno-sekretorni proizvodi Trichinella spiralis umanjuju produkciju MMP-9 u Dark Agouti pacova kojima je izazvan eksperimentalni autoimunski encefalomijelitis en ABSTRACT Matrix metalloproteinases (MMPs), are implicated in the pathogenesis of multiple sclerosis (MS) and in its animal model, experimental autoimmune encephalomyelitis (EAE). Our aim was to investigate whether amelioration of EAE in Dark Agouti (DA) rats, induced by Trichinella spiralis muscle larvae excretory-secretory products (ES L1), could be related to the level and activity of gelatinases, MMP-9 and MMP-2. Serum levels of MMP-9, MMP-2, NGAL/MMP-9, TIMP-1, and cytokines, evaluated by gel-zymography or ELISA, as well as gelatinases and TIMP-1 expression in the spinal cord (SC), were determined in: i) EAE induced, ii) ES L1-treated EAE induced animals. Milder clinical signs in ES L1-treated EAE induced DA rats were accompanied with lower serum levels of MMP-9 and NGAL/MMP-9 complex. However, the correlation between the severity of EAE and the level of serum MMP-9 was found only in the peak of the disease, with MMP-9/TIMP-1 ratio higher in EAE animals without ES L1 treatment. Lower expression of MMP-9 in SC of ES L1-treated, EAE induced rats, correlated with the reduced number of SC infiltrating cells. In SC infiltrates, in the effector and the recovery phase, production of anti-inflammatory cytokines IL-4 and IL-10 was higher in animals treated with ES L1 prior to EAE induction, compared to untreated EAE animals. Reduced expression of MMP-9 in SC tissue, which correlated with the reduced number of infiltrating cells, might be ascribed to regulatory mechanisms, among which is IL-10. Sažetak Trichinella spiralis, excretory secretory antigen, autoimmunity 1552099 10.1016/j.exppara.2021.108112 2022-05-25T17:37:54.299Z 44 yes 46 Fabrizio Bruschi Universita di Pisa, Department of Translational Research, N.T.M.S., Medical School, Italy Ljiljana Sofronić-Milosavljević University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia doktor medicinskih nauka person 0000-0002-2721-125X Nataša Ilić University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia doktor bioloških nauka person 0000-0001-6738-7033 Barbara Pinto Universita di Pisa, Department of Translational Research, N.T.M.S., Medical School, Italy person Alisa Gruden-Movsesijan University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia doktor bioloških nauka person 0000-0002-3061-5474 application/pdf 10268 https://phaidrabg.bg.ac.rs/o:25684 no yes 1 70 92000001 11A29 11A2904 Experimental Parasitology 225 Elsevier 2021