o:25575 Mesenchymal stromal cells from healthy and inflamed human gingiva respond differently to porphyromonas gingivalis Mezenhimalne stromalne ćelije, poreklom od zdrave i inflamirane gingive čoveka, različito reaguju na Porphiromonas gingivalis en ABSTRACT Gingiva-Derived Mesenchymal Stromal Cells (GMSCs) have been shown to play an important role in periodontitis. However, how P. gingivalis, one of the key etiological agents of the disease, affects healthy (H)- and periodontitis (P)-GMSCs is unknown. To address this problem, we established 10 H-GMSC and 12 P-GMSC lines. No significant differences in morphology, differentiation into chondroblasts and adipocytes, expression of characteristic MSCS markers, including pericyte antigens NG2 and PDGFR, were observed between H- and P-GMSC lines. However, proliferation, cell size and osteogenic potential were higher in P-GMSCs, in contrast to their lower ability to suppress mononuclear cell proliferation. P. gingivalis up-regulated the mRNA expression of IL-6, IL-8, MCP-1, GRO-α, RANTES, TLR-2, HIF-1α, OPG, MMP-3, SDF-1, HGF and IP-10 in P-GMSCs, whereas only IL-6, MCP-1 and GRO-α were up-regulated in H-GMSCs. The expression of MCP-1, RANTES, IP-10 and HGF was significantly higher in P-GMSCs compared to H-GMSCs, but IDO1 was lower. No significant changes in the expression of TLR-3, TLR-4, TGF-ß, LAP, IGFBP4 and TIMP-1 were observed in both types of GMSCs. In conclusion, our results suggest that P-GMSCs retain their pro-inflammatory properties in culture, exhibit lower immunosuppressive potential than their healthy counterparts, and impaired regeneration-associated gene induction in culture. All these functions are potentiated significantly by P. gingivalis treatment. Gingiva-Derived Mesenchymal Stromal Cells, Porphyromonas gingivalis, cell differentiation, gene expression, inflammation, tissue regeneration 1552099 10.3390/ijms23073510 2022-05-23T13:19:26.215Z 44 yes 46 Marina Bekić University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia 0000-0002-6945-4241 Miodrag Čolić University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia akademik person Dejan Bokonjić University of East Sarajevo, Medical Faculty Foča, Bosnia and Herzegovina person Bashkim Ismaili International Balkan University, Faculty of Dental Medicine, Skoplje, North Macedonia person Milan Marković University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia doktor medicinskih nauka-stomatolog person 0000-0001-8352-9003 Dejan Marković University of Belgrade, Faculty of Dental Medicine, Serbia person Miloš Duka University of Defense, Medical Faculty of the Military Medical Academy, Clinic for Stomatology, Belgrade, Serbia person Dušan Radojević University of Belgrade, Institute of Molecular Genetics and Genetic Engineering, Serbia person Mile Eraković University of Defense, Medical Faculty of the Military Medical Academy, Clinic for Stomatology, Belgrade, Serbia person Sergej Tomić University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia doktor bioloških nauka person 0000-0003-2570-1295 Jelena Đokić University of Belgrade, Institute of Molecular Genetics and Genetic Engineering, Serbia person Marina Radanović University of East Sarajevo, Medical Faculty Foča, Bosnia and Herzegovina person application/pdf 3780061 https://phaidrabg.bg.ac.rs/o:25575 no yes 16 70 92000001 11A29 11A2904 11A26 11A32 11A38 International Journal of Molecular Sciences 23 7 MDPI 2022