o:25316 Circulating MicroRNA Profiles as potential biomarkers for differentiated thyroid cancer recurrence Profili cirkulišućih mikroRNA kao potencijalni biomarkeri za rekurentni diferentovani tiroidni karcinom en ABSTRACT Context Circulating microRNAs (miRNAs) are emerging biomarkers of thyroid cancer. Objective This study sought to identify the profile of circulating miRNAs and its response to human recombinant TSH (rhTSH) in thyroid cancer patients with recurrent/persistent disease. Methods We obtained serum samples from 30 patients with differentiated thyroid cancer, 14 with recurrent/persistent disease and 16 with complete remission. We used next-generation sequencing to define the miRnomes along with a comprehensive quantitative PCR (qPCR) validation using 2 different platforms. We made a transversal study by comparing serum miRNA profiles of patients with or without recurrent/persistent disease and a longitudinal study looking at differences before and after rhTSH stimulation. Selected miRNAs were then studied in human thyroid cancer cell lines TPC-1, FTC-133, and OCUT-2 in response to TSH stimulation. Results We could not demonstrate any consistent differences in serum profiles of known miRNAs between patients with and without recurrent/persistent disease or before and after rhTSH stimulation. However, our sequencing data revealed 2 putative novel miRNAs that rise with rhTSH stimulation in the serums of patients with recurrent/persistent disease. We further confirmed by qPCR the upregulation of these putative miRNAs both in serums and in TSH-stimulated cells. We also show miRNAs that are good candidates for housekeeping genes in the serum of patients independently of the levels of TSH. Conclusions The present study does not provide evidence that known miRNAs can be used as circulating markers for recurrence of thyroid cancer. However, we suggest that novel miRNA molecules may be related to thyroid cancer pathogenesis. Sažetak microRNA, serum, thyroid cancer, recurrence, NGS, novel microRNA 1552099 10.1210/clinem/dgac009 2022-05-12T11:46:29.549Z 44 yes 46 Jelena Janković Miljuš University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia doktor biohemijskih nauka 0000-0002-2589-3306 María Augusta Guillén-Sacoto Hospital Universitario de Móstoles, Madrid E-28935, Spain person Garcilaso Riesco-Eizaguirre Hospital Universitario de Móstoles, Madrid, Spain person Pilar Santisteban Instituto de Investigaciones Biomédicas “Alberto Sols”, Consejo Superior de Investigaciones Científicas-Universidad Autónoma de Madrid, Spain person Mercedes Robledo Hereditary Endocrine Cancer Group, Spanish National Cancer Research Centre (CNIO) and Centro de Investigación Biomédica en Red de Enfermedades Raras (Ciberer), Madrid, Spain person Julia Ramirez-Moya Instituto de Investigaciones Biomédicas “Alberto Sols”, Consejo Superior de Investigaciones Científicas-Universidad Autónoma de Madrid, Spain person Jennifer Makiadi-Alvarado Instituto de Investigaciones Biomédicas “Alberto Sols”, Consejo Superior de Investigaciones Científicas-Universidad Autónoma de Madrid, Spain person León Wert-Lamas Instituto de Investigaciones Biomédicas “Alberto Sols”, Consejo Superior de Investigaciones Científicas-Universidad Autónoma de Madrid, Spain person application/pdf 6349 https://phaidrabg.bg.ac.rs/o:25316 no yes 1 70 92000001 11A29 11A2902 The Journal of Clinical Endocrinology & Metabolism 107 5 1280 1293 2022