o:25244 Rhizobacteria associated with Miscanthus x giganteus improve metal accumulation and plant growth in the flotation tailings Rizobakterije u vezi sa Miscanthus x giganteus poboljšavaju akumulaciju metala i rast biljaka gajenih na odlagalištu flotacione jalovine en ABSTRACT Purpose Flotation tailings represent an extremely unfriendly substrate for plant colonization due to toxic metal concentrations and marked macronutrient deficiencies. The perennial grass Miscanthus x giganteus J.M.Greef & Deuter ex Hodk. & Renvoize was successfully cultivated in this infertile substrate for two years. Our aim was to identify composition of its rhizosphere bacterial community and to analyze the effects of the selected rhizobacteria on plant growth, root development, metal and P uptake. Methods Using the cultivation-dependent method, 75 isolates were collected from the rhizosphere and six rhizobacterial strains were selected for further characterization based on morphological and biochemical differences. The plant rhizomes were inoculated with the consortium of rhizobacteria and cultivated in the flotation tailings substrate. Results Detected bacterial strains were characterized as metal-resistant and plant growth-promoting rhizobacteria (PGPR) because of their metal tolerance (NiCl2, Pb(C2H3O2)2, CuSO4, NaAsO2, MnCl2) and some or all of the plant growth-promoting (PGP) properties (indole-3-acetic acid and siderophore production, 1-aminocyclopropane-1-carboxylic acid deaminase activity and phosphate solubilization). PGPR mitigated the negative effects of high metal concentrations and macronutrient deficiency as shown by stimulated lateral roots development, increased root hair length, plant below and above ground biomass yield, higher plant P uptake and metal accumulation rate. Conclusions The isolated PGPR strains could be used in PGP-bacteria assisted phytoremediation of flotation tailings and metal polluted soils by M. x giganteus. Their PGP effects on various metal-tolerant target plant species in the respective substrate remain to be verified. Sažetak metals/metalloids, microorganisms, phytoremediation, PGPR, toxicity 1552099 10.1007/s11104-021-04865-5 2022-05-10T06:34:36.446Z 44 yes 46 Tamara Rakić University of Belgrade, Faculty of Biology, Department of Plant Ecology and Phytogeography, Serbia Mila Pešić University of Belgrade, Faculty of Biology, Department of Biochemistry and Molecular Biology, Serbia person Nikola Kostić University of Belgrade, Faculty of Biology, Department of Plant Ecology and Phytogeography, Serbia person Gordana Andrejić University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia doktor nauka-ekološke nauke person 0000-0002-5515-9321 Djordje Fira University of Belgrade, Faculty of Biology, Department of Biochemistry and Molecular Biology, Serbia person Željko Dželetović University of Belgrade, Institute for the Application of Nuclear Energy INEP, Serbia doktor biotehničkih nauka-oblast ratarstva person 0000-0001-9166-7094 Slaviša Stanković University of Belgrade, Faculty of Biology, Department of Microbiology, Serbia person Jelena Lozo University of Belgrade, Faculty of Biology, Department of Biochemistry and Molecular Biology, Serbia person application/pdf 87109 https://phaidrabg.bg.ac.rs/o:25244 no yes 1 70 92000001 11A02 11A0203 11A02 11A0212 11A29 11A2906 11A02 11A0204 Plant Soil 462 349 363 Springer Nature 2021