
<oai_dc:dc xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:oai_dc="http://www.openarchives.org/OAI/2.0/oai_dc/">
  <dc:creator>Jovanović, Marina I., 1989-, 57231881</dc:creator>
  <dc:title xml:lang="srp">Antimikrobni i citotoksični potencijal etanolnih ekstrakata odabranih predstavnika podfamilije Polygonoideae i njihov efekat u kombinaciji sa streptomicinom i doksorubicinom : doktorska disertacija</dc:title>
  <dc:description xml:lang="srp">Za ispitivanje antimikrobnog i citotoksičnog efekta odabrano je 8 biljaka podfamilije Polygonoideae od
kojih je napravljeno 12 etanolnih ekstrakata, od herbi (h) i rizoma (r): Polygonum aviculare (POAh,
POAr), Polygonum maritimum (POMh), Persicaria amphibia (PEAh), Persicaria maculosa (PEMh),
Persicaria lapathifolia (PELh, PELr), Persicaria hydropiper (PEHh, PEHr), Bistorta officinalis (BIOh,
BIOr) i Fagopyrum esculentum (FAEh). Svi testirani ekstrakti ispoljavaju umerenu antifungalnu
aktivnost, a POAh, POMh, PEAh, POAr, PEHr i BIOr dovode do obezbojavanja konidija. Antibakterijski
efekat je testiran prema većem broju G+ i G- bakterija pri čemu je BIOr pokazao najpotentnije
antimikrobno dejstvo. BIOr i PEMh pokazuju značajan antibiofilm i antiquorum sensing efekat prema
medicinski važnom patogenu Pseudomonas aeruginosa PAO1 i to preko modulacije aktivnosti LasR
receptora. Ovi ekstrakti i njihovi aktivni konstituenti, hinska i hlorogenska kiselina, katehin i kvercetin-
3-O-glukozid, inhibiraju sintezu violaceina kod Chromobacterium violaceum. U kombinaciji sa
streptomicinom BIOr ostvaruje različite tipove interakcija: antagonistički, indiferentni, aditivni i
sinergistički. Prilikom testiranja citotoksičnosti odabranih ekstrakata i doksorubicina (D) uočen je
sinergizam između P. aviculare i D na ćelijama hepatocelularnog adenokarcinoma (HepG2) i plućnog
adenokarcinoma (A549). Takođe, sinergizam između P. amphibia i D je detektovan na A549 ćelijama.
Sinergistički efekat se ostvaruje preko sledećih procesa: uvođenje ćelija u apoptozu, zaustavljanje
ćelijskog ciklusa, modelovanje aktivnosti antioksidativnih enzima i Keap1-Nrf2 signalnog puta. Na
normalnim ćelijama fibroblasta pluća (MRC-5) P. aviculare i D, P. amphibia i D kao i P. maculosa i D
ostvaruju antagonizam. Odsustvo toksičnosti PEMh i BIOr u nižim dozama, potvrđeno je in vivo testom
na modelu Danio rerio.</dc:description>
  <dc:description xml:lang="eng">were selected and 12 ethanol extracts were made. Herb (h) and rhizome (r) extracts were obtained from:
Polygonum aviculare (POAh, POAr), Polygonum maritimum (POMh), Persicaria amphibia (PEAh),
Persicaria maculosa (PEMh), Persicaria lapathifolia (PELh, PELr), Persicaria hydropiper (PEHh,
PEHr), Bistorta officinalis (BIOh, BIOr) and Fagopyrum esculentum (FAEh). All tested extracts
moderately inhibited the growth of selected fungi. POAh, POMh, PEAh, POAr, PEHr and BIOr leads to
discoloration of conidia. Antibacterial activity on different Gram positive and Gram negative strains has
been evaluated. BIOr showed the most potent antibacterial activity. Also, BIOr and PEMh exhibited
significant antibiofim and antiquorum sensing effects against medically important pathogen,
Pseudomonas aeruginosa PAO1, through the modulation of LasR receptor activity. Both extracts as well
as their active constituents, chinic acid, chlorogenic acid, catechin and quercetin-3-O-glucoside, inhibited
violacein synthesis in Chromobacterium violaceum. The BIOr engaged in various interactions with
streptomycin: synergic, addictive, indifferent and antagonistic. Cytotoxicity testing of selected extracts
and doxorubicin (D) revealed a synergistic relationship between P. aviculare and D, in hepatocellular
adenocarcinoma (HepG2) and pulmonary adenocarcinoma cells (A549). Also, synergism between P.
amphibia and D, was detected on A549 cells. The observed synergisms could be linked to the interference
with apoptosis, cell cycle, antioxidant enzymes expression and Keap1-Nrf2 pathway. On normal lung
fibroblast cells (MRC-5) P. aviculare and D, P. amphibia and D as well as P. maculosa and D exerts an
antagonistic effect. In vivo assay on the Danio rerio model shows no signs of toxicity at lower tested
concentrations for PEMh and BIOr.</dc:description>
  <dc:description xml:lang="srp">Biologija - Mikrobiologija, Genotoksikologija / Biology - Microbiology, Genotoxicology  
Datum odbrane: 03.07.2020. </dc:description>
  <dc:format>129 str.</dc:format>
  <dc:format>5694730 bytes</dc:format>
  <dc:identifier>https://phaidrabg.bg.ac.rs/o:22560</dc:identifier>
  <dc:identifier>cobiss:20837641</dc:identifier>
  <dc:identifier>thesis:7609</dc:identifier>
  <dc:language>srp</dc:language>
  <dc:date>2020</dc:date>
  <dc:subject xml:lang="eng">OSNO - Opšta sistematizacija naučnih oblasti, Mikrobiologija. Bakteriologija</dc:subject>
  <dc:subject xml:lang="srp">OSNO - Opšta sistematizacija naučnih oblasti, Mikrobiologija. Bakteriologija</dc:subject>
  <dc:subject xml:lang="srp">Polygonoideae, antimikrobni efekat, antibiofilm efekat, quorum sensing, Danio rerio,doksorubicin, apoptoza, zaustavljanje ćelijskog ciklusa, antioksidativni enzimi, Keap1-Nrf2 signalni put</dc:subject>
  <dc:subject xml:lang="srp">Polygonoideae, antimicrobial effect, antibiofilm effect, quorum sensing, Danio rerio,doxorubicin, apoptosis, cell cycle arrest, antioxidative enzymes, Keap1-Nrf2 signaling pathway</dc:subject>
  <dc:subject xml:lang="eng">579.61:615.322(043.3)</dc:subject>
  <dc:rights>http://creativecommons.org/licenses/by-nc-sa/2.0/at/legalcode</dc:rights>
  <dc:contributor>Mitić-Ćulafić, Dragana, 1960-, 12551527</dc:contributor>
  <dc:contributor>Nikolić, Biljana, 1971-, 12824935</dc:contributor>
  <dc:contributor>Jasnić, Nebojša, 1979-, 12937319</dc:contributor>
  <dc:contributor>Svirčev, Emilija, 1979-, 57739017</dc:contributor>
  <dc:type>info:eu-repo/semantics/bachelorThesis</dc:type>
</oai_dc:dc>