o:22551 Раманова спектроскопија фармаколошки активних супстанци и биокатализатора докторска дисертација Raman spectroscopy of pharmacologically active compounds and biocatalysts : doctoral dissertation sr U ovoj disertaciji Ramanova spektroskopija korišćena je za sveobuhvatnu analizu primarnih mezenhimskih matičnih ćelija, poreklom iz periodoncijuma, mekog vezivnog tkiva koje spaja koren zuba za alveolarnu kost i ima ulogu u održavanju mineralizacije i ublažavaanja sila koje se javljaju žvakanjem... In this dissertation, the utilization of Raman spectroscopy for comprehensive analysis of primary mesenchymal stem cells from periodontal ligament, a soft connective tissue which anchors tooth root for the alveolar bone, was demonstrated. Since every cell has the unique Raman spectrum, Raman spectroscopy was used with the purpose to gain the insight in intrinsic biochemical prole on a single cell level. Mesenchymal stem cells from periodontal ligament were stimulated to dierentiate towards osteogenic, chondrogenic, and adipogenic lineages. These dierentiated cells were investigated by means of Raman spectroscopy as well. Microscopic glass cover-slips were used as substrates due to its cost-eectiveness and common use in laboratories, which is important for its clinical application. On the other hand, glass has strong Raman response, but it was demonstrated that the analysis was possible by taking into account certain spectral region in which its inuence was eliminated. In all sample groups, the same type of behavior was noticed, manifested as the increase of relative intensity Raman band of proteins/lipids, and the decrease of relative intensity of nucleic acids Raman bands. Further, the extensive statistical analysis in the form of principal component analysis was performed, revealing signi- cant groupings of cells with the same spectral features, which conrmed previously obtained results. Despite the heterogeneity of primary mesenchymal stem cells and their dierentiated lineages, it was proved that micro-Raman spectroscopy could adequately distinguish cell status, which is important for its clinical and medical use... Tehnološko-inženjerstvo - Biohemijsko inženjerstvo i biotehnologija /Technological engineeringResearch area: Biochemical engineering and biotechnology Datum odbrane: 26.06.2020. Ramanova spektroskopija, mezehnimske matične ćelije, analiza pojedinačnih ćelija, polimorfizam, međumolekularne vibracije Raman spectroscopy, Mesenchymal stem cells, Single cell analysis, Polymorphism,Intermolecular vibrations yes 20693769 91552101 7605 91552100 20693769 2020-09-25T13:17:41.053Z 45 no 46 mentor Jasmina J., 1985-, 57142281 Lazarević 2019 63 mentor Branko, 1957-, 12474215 Bugarski 2019 63 član komisije Zorica, 1969-, 12781159 Knežević-Jugović 2019 63 član komisije Diana, 1956-, 1665383 Bugarski 2019 63 član komisije Zoran V., 1952-, 8073575 Popović 2019 82 стр. 19570754 http://phaidrabg.bg.ac.rs/o:22551 no yes 4 2020-09-25T13:17:41.320Z 70 11 1066841 1066935 1066942 Ramanova spektroskopija, mezehnimske matične ćelije, analiza pojedinačnih ćelija, polimorfizam, međumolekularne vibracije Raman spectroscopy, Mesenchymal stem cells, Single cell analysis, Polymorphism,Intermolecular vibrations 543.42:577.27(043.3) 1738 11A41 2019